High speed countercurrent chromatography is based on a unidirectional fluid dynamic equilibrium system, utilizing the directionality of the helical tube and the two-dimensional centrifugal force field generated by synchronous planetary motion to form a unidirectional fluid dynamic equilibrium. When the spiral tube rotates slowly, the two-phase solvents are distributed from one end to the other; When using a certain phase as the mobile phase to elute from one end to the other, the retention value of the other phase in the spiral tube is about 50%, but this retention amount decreases with the increase of the mobile phase flow rate, resulting in a decrease in separation efficiency. When the speed of the spiral tube accelerates to the critical range, the two phases will separate along the length of the spiral tube, with one phase occupying a section of the head end (head end) and the other phase occupying a section of the tail end (tail end). High speed counter current chromatography utilizes this special fluid dynamics phenomenon to effectively contact, mix, distribute, and transfer immiscible two-phase solvents (stationary phase and mobile phase) in a helical tube. The stationary phase is distributed in a relatively uniform manner in a spiral tube made of polytetrafluoroethylene. The mobile phase passes through the stationary phase at a certain speed and is eluted sequentially according to the different distribution coefficients of the separated substances to obtain separation. The faster the instrument speed, the more fixed phase is retained, the better the separation effect, and greatly improves the separation speed.
Product Features
No solid filler pollution: Avoid sample and filler adsorption, irreversible adsorption or degradation, with high recovery rate (usually>90%).
High separation efficiency: The strong centrifugal force generated by high-speed rotation enhances the distribution process, enabling fast and effective separation.
Large sample loading capacity: The preparation model can process samples ranging from milligrams to grams in a single operation, making it suitable for industrial scaling up.
Wide adaptability: The solvent system can be flexibly selected according to the polarity of the sample, and is compatible with acidic, alkaline, and thermosensitive samples.
The maintenance of high-speed countercurrent chromatography requires special attention to column cleaning, circuit interface maintenance, and battery maintenance. The following are specific maintenance points:
Column maintenance
After each experiment, it is necessary to rinse the chromatography column with a dedicated mobile phase to prevent residual substances from affecting subsequent experiments. Check the connection between the pump and the injection module for looseness every week to avoid excessive elution deviation.
Circuit interface maintenance
If the circuit interface oxidizes, it can be lightly sanded with fine sandpaper and coated with conductive paste to prevent rust. When not in use for a long time, the power adapter needs to be disconnected and the circuit activated by powering on for 30 minutes every month.
battery maintenance
Long term disuse requires fully charging and connecting the power adapter for 30 minutes to activate the battery, in order to avoid damage caused by low power. Before starting up, it is necessary to calibrate the instrument with a standard sample for 2 hours to ensure that the elution deviation is less than 1%.