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Shanghai Shujun Instrument Equipment Co., Ltd
Understanding the functional characteristics of each component of the Biotage column passing machine is essential for better use
Date: 2025-12-01Read: 0
Biotage column passing machine is a key platform for achieving efficient and automated purification of compounds in modern drug development, natural product separation, and biochemical laboratories. It is widely used in scenarios such as combinatorial chemistry library construction, metabolite separation, and intermediate purification. Its core advantages include multi-channel parallelism, intelligent gradient elution, and real-time detection feedback, significantly improving separation efficiency and sample recovery rate. The performance of the Biotage column passing machine depends on the collaborative integration of multiple precision modules.
1、 Multi channel solvent transfer pump
Adopting a quaternary or multicomponent high-pressure gradient pump, with a flow rate range of 0.1-50mL/min and an accuracy of ± 0.5%, it can simultaneously transport aqueous phase, organic phase, and buffer solution. Support linear/step gradient programming to ensure the separation of different polar compounds under optimal elution conditions. Some models are equipped with low diffusion flow paths to reduce peak broadening and improve resolution.
2、 Automatic sampler and sample tray
96 well or deep hole plate compatible design: capable of processing tens to hundreds of samples at a time, supporting SBS standard plate types;
Accurate sampling needle: equipped with liquid level detection, anti-collision and cleaning functions to avoid cross contamination;
Temperature controlled sample chamber (4-40 ℃): protects the stability of thermosensitive compounds.
3、 High throughput chromatography column switching valve group
Integrated with multiple six or ten way valves, it can automatically switch between different specifications of chromatographic columns (such as C18, silica gel, amino based columns), achieving positive and negative phase switching, column series connection or bypass cleaning. The valve body is made of high-pressure resistant PEEK or stainless steel material, with a dead volume of less than 10 μ L, ensuring peak symmetry.
4、 Multi wavelength UV visible detector
Dual wavelength or multi wavelength synchronous detection (such as 214nm, 254nm, 280nm): balancing the response of peptide bonds, aromatic rings, and conjugated systems;
The optical path length of the flow cell can be selected (1-10mm) to adapt to samples of different concentrations and avoid signal saturation;
Real time baseline correction and peak integration: enhance the detection capability of low abundance components.
5、 Fraction collector
Collection triggered by time, threshold, or peak: supports multiple containers such as test tubes, 96 well plates, deep well plates, etc;
Position memory and error prevention design: avoid missing or misplacement;
Inert gas protection interface (optional): prevents degradation of oxidation sensitive substances.
6、 Intelligent control software
Graphic method editing interface: drag and drop settings for gradient, flow velocity, detection, and collection parameters;
Method library management and audit tracking: compliant with GLP/GMP standards;
Remote monitoring and data export: supports CSV and PDF report generation, seamlessly integrates with LIMS system.