Fluorescence intensity meterBased on photoluminescence phenomenon: When a fluorescent substance is irradiated with excitation light (such as xenon arc lamp, tungsten bromide lamp, etc.), it absorbs energy and transitions to the excited state, and then emits longer wavelength characteristic fluorescence when returning to the ground state. The instrument achieves quantitative analysis of substance concentration or characteristics by measuring the intensity of fluorescence, combined with standard curve method or direct comparison method.

　　Fluorescence intensity meterPrecautions for operation:

Standard curve method:

Prepare a series of standard solutions, measure their fluorescence intensity, and draw a standard curve with fluorescence intensity as the y-axis and concentration as the x-axis.

Subsequent sample analysis needs to be conducted under the same conditions.

Temperature control:

The fluorescence intensity is significantly affected by temperature, and the temperature change during testing should be controlled within ± 3 ℃.

The advanced fluorescence meter is equipped with a liquid tank cooling device to maintain a constant temperature.

PH adjustment:

For weak acid or weak base fluorescent substances, the pH value of the solution will affect the molecular ionization equilibrium, thereby changing the fluorescence intensity.

The optimal pH range needs to be determined through conditional testing.

Fluorescence quencher:

Halogen ions, heavy metal ions, dissolved oxygen, etc. may cause fluorescence quenching, and it is necessary to remove oxygen or avoid using quenchers in the experiment.