High performance liquid chromatographyBy using a static motor control circuit, the automatic feedback tracking function of the motor control signal is achieved, effectively improving the accuracy of motor control and ensuring the reliability and stability of the pump.
Convenient and easy-to-use flow and pressure calibration functions ensure high precision and stability of the instrument.
Two gradient running program designs are available: linear gradient and step gradient.
Programmable solvents and compression factors that can be automatically compensated.
Complete pre cleaning function, facilitating quick solvent replacement.
Universal sample cell design, easy to replace between trace and constant cells.
Deuterium lamps are easy and fast to replace, and can be used without calibration.
Gradient system hardware inspection:
Pump performance verification:
Check if the proportional valve is malfunctioning or if the sealing gasket is damaged, and replace the faulty component.
Exclude air bubbles from the pump: Rinse through the Purge valve or use a specialized syringe to extract the bubbles.
Mobile phase treatment:
Ensure that the solvent is fully degassed to avoid pressure fluctuations caused by bubbles.
Use HPLC grade solvents to prevent proportional deviations caused by volatilization or contamination.
Gradient program optimization:
Initial gradient setting:
Use a wide range gradient (such as 5-95% acetonitrile) to quickly evaluate peak distribution, and then make targeted adjustments.
Adjust the initial ratio by averaging: If the retention time of the target peak is 10 minutes and the corresponding solvent ratio is 30%, the initial ratio can be set to (5+30)/2=17.5%.
Local gradient optimization:
For difficult to separate peak groups, reduce the gradient rate to 1/2 or 3/4 of the original rate and prolong the elution time.
If the separation is too large, the gradient termination ratio can be appropriately increased to shorten the analysis time.