Abstract:

This study used ultraviolet spectrophotometry to investigate the determination conditions of selenium content in Enshi Wujiatai tribute tea samplespHValue, dosage of chelating agent ortho phenylenediamineEDTADosage, complexation time, and temperature5The impact of various influencing factors on the measurement results. Under optimal conditions, the standard curve0~1.92μg/mLThere is a linear relationship within the concentration range, and the regression equation isy=0.50089x+0.00957，R2=0.99939The results of the sample determination showed that the average content of selenium in Wujiatai tribute tea was2.0740μg/gMeets the standards for selenium rich tea.

Selenium is known as the "spark of life" and plays multiple biological roles in the human body, including antioxidant, immune enhancement, prevention and treatment of cancer, and prevention of cardiovascular disease. It plays a crucial role in maintaining numerous physiological and metabolic processes. However, due to the inability of the human body to synthesize selenium on its own, people need to obtain this important trace element from their diet. Therefore, an appropriate intake of selenium is crucial for maintaining human health, as both deficiency and excess may have adverse effects on human health. Plants play a crucial role in the human body's acquisition of selenium, as they act as mediators to transfer selenium from the soil to the interior of the body. Among numerous plants, tea trees exhibit significant selenium enrichment ability, effectively absorbing and accumulating selenium from the soil, and storing it inside the plant. In low selenium areas, the average selenium content in tea is several to tens of times that of corn, rice, and wheat, making selenium rich tea a relatively inexpensive selenium supplement food.

ultraviolet-Visible spectrophotometry can meet the needs of selenium element determination, and is widely used in the field of food testing due to its advantages in sensitivity, accuracy, ease of operation, cost-effectiveness, and applicability. This article optimizes and improves the determination method by setting different testing conditions, in order to explore the determination of selenium content in teaThe best conditions.