Insoluble particle detectorIt is a high-tech precision instrument specifically designed to detect parameters such as the quantity, size, and distribution of insoluble particles in liquids. Its working principle is mainly based on the principle of light scattering. When the laser beam passes through the liquid sample to be tested, insoluble particles in the liquid scatter light, generating a light scattering signal. These signals are captured by precision detectors and converted into electronic data through the internal circuitry of the instrument. By analyzing these data, the instrument can accurately determine the size and quantity of particles. In addition, the insoluble particle detector may also use various working principles such as photoresist method, microscopic counting method, electrophoresis method, and resistance method for detection.
Insoluble particle detectorOperation steps:
1、 Preparation work
Environmental inspection:
Ensure that the testing environment meets the requirements: temperature 10-40 ℃, humidity ≤ 85%, and no strong electromagnetic interference sources (such as mobile phones and large motors).
Avoid direct sunlight or vibration sources, and keep the workbench clean and dry.
Instrument inspection:
Check if the appearance of the instrument is intact and if the power and data cables are securely connected.
Confirm that components such as sensors, injection needles, and mixers are not blocked or damaged.
Check the status of the filter and replace the pre filter if necessary (it is recommended to replace it once a week).
Sample preparation:
The sample needs to be filtered through a filter membrane (such as a 0.45 μ m or 0.22 μ m filter membrane) to remove large particle impurities.
Ensure that the sample container is clean and avoid contamination.
Prepare a sufficient amount of sample (usually 20-100ml) according to testing standards such as the Chinese Pharmacopoeia and USP.
Standard material preparation (for calibration):
Use a standard particle suspension with a known particle size distribution (such as polystyrene microspheres) for calibration.
Shake the standard substance well before calibration to avoid precipitation.
2、 Startup and initialization
Connect the power supply:
Insert the instrument power cord into a stable power socket (220V ± 10%) and turn on the power switch.
Wait for the instrument self-test to complete (usually displaying a startup interface or indicator light changing from red to green).
System preheating:
Preheat for 5-10 minutes according to the instrument requirements to ensure the stability of the sensor.
During the preheating period, pipeline flushing can be carried out (using purified water or injection water).
Software login and parameter settings:
Open the supporting software, enter your username and password to log in.
Set detection parameters:
Detection mode: Select "Manual" or "Automatic".
Detection channel: Select the appropriate channel based on the sample type (such as photoresist method or microscopic counting method).
Particle size range: Set the particle size range to be detected (such as 2-100 μ m).
Detection volume: Enter the sample volume (such as 10ml or 25ml).
Mixing speed: Set the mixer speed (to avoid generating bubbles).
Injection speed: Adjust the movement speed of the injection needle according to the instrument model.
3、 Calibration operation
Blank calibration:
Rinse the pipeline 3 times with purified water or injection water to eliminate bubbles and residues.
Run blank detection and record background values (ensuring particle count ≤ standard requirements, such as ≤ 10 particles ≥ 10 μ m per milliliter).
Calibration of standard substances:
Inject standard particle suspension and run for testing.
Compare the instrument detection results with the standard values and adjust the calibration coefficients (such as slope and intercept).
Repeat calibration until the error is ≤ 5%.
4、 Sample testing
Sample injection:
Pour the sample into a specialized container (such as a silica glass bottle) to avoid the formation of bubbles.
Place the container on the mixer and adjust the position of the injection needle (at a distance of ≥ 1cm from the liquid level).
Start detection:
Click "Start Detection" in the software, and the instrument will automatically complete the following steps:
Stir the sample until uniform.
Inject a quantitative sample into the injection needle.
The sample is subjected to sensors to detect the number and size of particles.
Display the detection results (such as the number of particles ≥ 10 μ m and ≥ 25 μ m per milliliter).
Data recording and storage:
After the detection is completed, the software automatically generates a report (including particle size distribution map and statistical data).
Save the report to the designated folder or print a hard copy.
5、 Cleaning and shutdown
Pipeline cleaning:
Rinse the pipeline three times with purified water or injection water to eliminate residual samples.
For viscous samples (such as injection solutions and liposomes), organic solvents (such as isopropanol) should be used for cleaning.
Shutdown operation:
Click 'Exit' in the software to turn off the instrument power.
Disconnect the power cord and cover it with a dust cover.
Waste disposal:
Dispose of used samples and cleaning solutions according to laboratory regulations (such as high-temperature sterilization for medical waste).