Multi angle laser light scattering instrumentIt is a high-precision analytical instrument based on the principle of laser scattering, which directly measures parameters such as absolute molecular weight, molecular size, and distribution of polymer materials, biomolecules, and nanoparticles by detecting changes in scattered light intensity from multiple angles.

Testing process:

1. Sample preparation: Dissolve the sample in a low scattering solvent, filter to remove dust and large particle impurities, and measure the refractive index increment (dn/dc) between the solvent and the sample, which is a key prerequisite parameter for calculating molecular weight;

2. Light scattering signal acquisition: When the laser passes through the sample cell, large molecules/particles produce elastic scattering. Detectors at different angles synchronously record the scattered light intensity, while RI/UV detectors record the sample concentration;

3. Data fitting calculation: eliminate the influence of angle and concentration on scattered light intensity through Zimm plot method, substitute into Rayleigh scattering formula, and solve for absolute molecular weight (Mw); Calculate the rotation radius (Rg) based on the intensity difference of scattered light at different angles, and then infer the molecular conformation;

4. Result output: The software automatically generates data such as molecular weight distribution curve, Rg distribution, A ₂ value, etc., which intuitively reflects the uniformity and thermodynamic properties of the sample.

　　Multi angle laser light scattering instrumentBy synchronously collecting scattered light signals from multiple angles and combining them with concentration data, molecular size, conformation, and aggregation state information can be obtained. It is widely applicable to biological macromolecules, synthetic polymers, nanoparticles, and other samples. It is often used in online combination with SEC (volume exclusion chromatography), FFF (field current separation) and other technologies, and is a key instrument for scientific research and industrial quality control.