1、 Application and operational skills in the field of biology

Cell lysis and DNA/protein extraction

Application:ultrasonic homogenizerBy utilizing the cavitation effect to break down animal and plant cells, viruses, or bacteria, releasing intracellular substances such as proteins and nucleic acids, it is commonly used in genetic engineering and proteomics research.

Operation skills:

Choose the appropriate probe: Small diameter probes (such as 3mm) are suitable for trace samples, while large diameter probes (such as 10mm) are suitable for large volume samples.

Control parameters: Set the power to 30% -50% and wait for 1-3 minutes to avoid excessive fragmentation and deactivation of biomolecules.

Low temperature operation: The sample needs to be pre cooled to 4 ℃ and used in conjunction with an ice bath to prevent thermal damage.

Virus and vaccine preparation

Application: Crushing virus cells, extracting antigens for vaccine development.

Operation skills:

Optimize amplitude: Control the amplitude within 50% -70% to ensure even fragmentation of virus particles.

Short term multiple processing: 10-15 seconds each time, with a 30 second interval, repeated 3-5 times to avoid local overheating.

Case Study

A certain biological laboratory used an ultrasonic homogenizer to crush Escherichia coli and extract intracellular enzymes, with a crushing rate of over 95% and an enzyme activity retention rate of over 80%.

2、 Application and operational skills in the field of chemistry

Dispersion and emulsification of nanomaterials

Application: Preparation of nano lotion and dispersion of nano particles (such as carbon nanotubes, metal oxides) to improve the uniformity and stability of materials.

Operation skills:

Power and time: Set the power at 40% -60% and the time at 5-10 minutes, and use it in conjunction with a mixer.

Solvent selection: Use polar solvents (such as ethanol, water) to enhance the cavitation effect.

Chemical reaction acceleration

Application: Promote organic synthesis reactions (such as esterification and coupling reactions) and shorten reaction time.

Operation skills:

Temperature control: The temperature of the reaction system should not exceed 60 ℃ to avoid side reactions.

Intermittent processing: pause for 1 minute every 2 minutes to prevent local overheating.

Case Study

A certain chemical enterprise: usingultrasonic homogenizerDisperse graphene to prepare conductive ink, reducing particle size from 10 μ m to 200nm and increasing conductivity by three times.

3、 General operating skills and precautions

Equipment Calibration

Before each use, check if the probe is worn, and calibrate the amplitude and power to standard values.

Sample pretreatment

Remove large particle impurities to avoid clogging the probe.

safety protection

Wear goggles and earplugs to avoid damage from ultrasonic radiation and noise.

Cleaning and maintenance

Clean the probe immediately after use to avoid residue solidification.