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Breaking through the bottleneck of AD treatment: Jess system assists in the study of blood-brain barrier mechanism and validation of nanotherapy
Date: 2025-12-09Read: 1
Alzheimer's disease (AD), as a neurodegenerative disease that urgently needs to be overcome, is characterized by abnormal deposition of β - amyloid protein (A β) in the brain, and dysfunction of the blood-brain barrier (BBB) is a key factor leading to obstruction of A β clearance. Recently, West China Hospital of Sichuan University has collaborated with the nationalThe international team has published a groundbreaking study in Signal Transduction and Targeted Therapy (IF=52.7), proposing a novel nanotherapy strategy - regulating the transport pathway of LRP1 receptors on the BBB through A ₄₀ - POS nanocarriers, achieving rapid clearance of A β and long-term recovery of cognitive function.
01. Three major breakthroughs in research
This study achieved three key breakthroughs: clearing nearly 45% of A β in the brains of AD model mice within 2 hours, repairing BBB transport function, and maintaining cognitive improvement effects for 6 months.Behind this series of breakthrough conclusions, precise and reliable experimental technology support is indispensable - the ProteinSimple Jess automated digital Western blot system. Its core application in verifying the purity of brain separation components (Figure S12) provides key data guarantee for the rigor of the research mechanism.
02. Core technical support:Key Applications of Jess System in Experiments
In the study of AD pathological mechanisms, precise separation of cerebral blood vessels from parenchymal tissue is a prerequisite for analyzing BBB transport function. The research team used the dextran density gradient method to separate the vascular and parenchymal components of mouse brain tissue. Specific markers were used to verify the purity of the separation, ensuring the accuracy of subsequent experimental data on A β transport and receptor expression. This critical verification step was completed through the Jess system.
03. Data interpretation: Accurate verification of purity of separated components
(1) Experimental design:
Measure the expression levels of endothelial cell marker (CD31), pericyte marker (CD146), and neuronal marker (NeuN) separately for the separated cerebral vascular and parenchymal components.
(2) Jess system detection result:
In the vascular components, the band signals of CD31 and CD146 are strong, and the peak area of grayscale values is significantly higher than that of the parenchymal components, indicating complete separation of vascular tissue;
In the substantive components, the signal of the neuronal marker NeuN is dominant, while the signal of the vascular marker is weak, indicating that there was no significant cross contamination during the separation process;
The strip image is clear without trailing, and the gray value quantitative data has good repeatability (n ≥ 3), laying the foundation for accurate quantification of proteins such as LRP1 and PACSIN2 in the future.
04. Technical advantages of Jess system: why is it the preferred tool for this study?
(1) Automated operation to avoid human error
Traditional Western blot requires manual completion of multiple steps such as gel preparation, electrophoresis, membrane transfer, and incubation, while the Jess system achieves full process automation, reducing differences in experimental personnel operations and ensuring data stability - which is crucial for experiments with small sample sizes and complex processing such as brain separation.
(2)Micro sample detection, suitable for precious tissues
The brain tissue of AD model mice is scarce, and the sample size of isolated vascular/parenchymal components is extremely small. The Jess system only requires 3 μ L of sample to complete the detection, which is much lower than the sample requirement of traditional Western, and maximizes the use of valuable experimental materials.
(3) Accurate quantification, surpassing qualitative analysis
Through Compass for Simple Western software, precise calculation of peak area of grayscale values is achieved, providing quantitative data instead of traditional Western qualitative/semi quantitative results, meeting the strict requirements of "component purity quantification verification" in research.
(4) High resolution separation, clear identification of biomarkers
Using a 12-230 kDa separation module, different molecular weight biomarkers such as CD31 (~135 kDa), CD146 (~75 kDa), and NeuN (~50 kDa) were successfully distinguished with high band resolution and no overlapping interference.
05. Summary and Prospect
The ProteinSimple Jess automated digital Western blot system, with its technical advantages of "micro sample adaptation, automated operation, and precise quantification," has become an important technical support in the breakthrough research of Alzheimer's disease. The purity verification of brain isolated components completed in this study provides a reliable data basis for the mechanism analysis of the entire study.
In the research of neurodegenerative diseases, sample scarcity, complex mechanisms, and high quantitative requirements are common challenges, and the Jess system precisely addresses these pain points. With the development of emerging fields such as nanomedicine and vascular targeted therapy, the Jess system will continue to provide precise and reliable technical support for neuroscience research, disease mechanism analysis, and innovative therapy validation, helping more breakthrough achievements such as A ₄₀ - POS nanotherapies to be implemented and bringing new hope for the treatment of difficult to treat diseases such as AD.
* This article is reproduced from "ProteinSimple" WeChat official account
▍About ProteinSimple
ProteinSimple is a protein analysis brand under Bio Techne Group (NASDAQ: TECH), a publicly traded company on NASDAQ in the United States. Making protein analysis simpler has always been ProteinSimple's vision. ProteinSimple has long been committed to developing and producing innovative protein analysis tools that are more precise, faster, and more sensitive, including protein charge characterization, protein purity analysis, quantitative detection of protein post-translational modifications, protein immunoassay technologies such as digital Western Blot and high-sensitivity microfluidic multiplex ELISA, to help scientists in fields such as biopharmaceuticals, cell and gene therapy, biomedical and life sciences solve protein analysis problems, accelerate drug development efficiency, and deeply analyze the relationship between proteins and diseases.As the agent of ProteinSimple in the East China region (Jiangsu, Zhejiang, Shanghai, Anhui, Shandong), Shanghai Haokuan will provide ProteinSimple's instruments and equipment to everyone.
▍Regarding Shanghai Haokuan:
Shanghai Haokuo Scientific Equipment Co., Ltd. was established in 2019 and is headquartered in Shanghai. It is a professional service provider of laboratory equipment/consumables and analytical instruments. The company is committed to providing high-tech professional equipment and technical consulting services to customers in the fields of biology, medicine, physical property testing, chemical analysis, food, industrial production, and other related fields at home and abroad.