Compared to traditional manual column passing,Automatic column passing machineThe advantages are very significant:

1. High repeatability of separation: parameters such as flow rate, elution gradient, and sample loading volume are controlled by the program to avoid manual errors and ensure consistent experimental results across different batches.

2. Liberate manpower: No need for manual monitoring throughout the entire process, enabling unmanned operation and improving laboratory work efficiency.

3. Higher separation accuracy: precise gradient elution and real-time detection can effectively separate components with similar properties, especially suitable for the purification of trace target substances.

4. Reduce solvent waste: Accurately collect based on detection signals to avoid the collection of ineffective eluents, reduce organic solvent consumption and waste liquid treatment costs.

　　Automatic column passing machineThe core process is as follows:

1. Sample loading: Load the mixture sample into a chromatographic column filled with adsorbent. Different compounds in the sample move at different rates in the column due to differences in adsorption capacity.

2. Separation process: Different compounds are separated in the column by gradually changing the composition or concentration of the mobile phase (solvent). Compounds with weak adsorption capacity flow out first, while compounds with strong adsorption capacity flow out later.

3. Elution and collection: Compounds adsorbed on the column are eluted and separated from the column, and collected by the collection system to obtain purified target compounds.