Different types of cells have different requirements for culture conditions, so it is necessary to choose a suitable 87050 cell culture tube. For example, adherent cells need to choose those with good air permeability, while suspended cells can choose those with good sealing properties; Different experimental requirements have different requirements for specifications, materials, bottle cap types, etc. Therefore, when choosing, it is necessary to fully consider the experimental requirements.

The determination steps of 87050 cell culture tube mainly include the following aspects:

1. Preparation stage

-Select appropriate culture tubes: Choose cell culture tubes of appropriate size and type according to experimental requirements, such as common centrifuge tubes, cell culture bottles, etc.

-Cleaning and sterilization: Ensure that the culture tube is clean and free of contamination, and can be subjected to high-temperature and high-pressure sterilization or dry heat sterilization treatment.

-Preparation of reagents: including cell culture medium (preheated to an appropriate temperature, usually selecting a portion of the culture medium preheated to save time and avoid protein degradation), trypsin (used for digesting cells), serum (providing the necessary nutrients for cell growth), etc.

2. Inoculate cells

-Cell counting: If accurate control of cell seeding volume is required, cell counting should be performed first, using a cell counting plate or automatic cell counter to determine the density of the cell suspension.

-Inoculate cells: Transfer an appropriate amount of cell suspension into a culture tube and gently shake to evenly distribute the cells.

3. Cultivation process

-Put into the incubator: Place the culture tube inoculated with cells into the incubator and set the appropriate temperature (usually 37 ° C), humidity (usually 40-60 ° C), and CO? Concentration (usually 5 ° C).

-Regular observation: Observe the growth status of cells at regular intervals every day, including cell morphology, adhesion, and contamination. It can be observed directly with the naked eye or examined in more detail using a microscope.

4. Cell processing and collection

-Fluid replacement: Regularly replace fresh culture medium based on cell growth and experimental needs to provide sufficient nutrition and remove metabolites and dead cells.

-Passage: When cells reach a certain density (usually 70-80 fusion degree), passage is required. Use trypsin to digest the cells, causing them to detach from the bottom of the culture tube, then add fresh culture medium to terminate digestion, blow them into a single-cell suspension, and transfer them to a new culture tube for further cultivation.

-Collect cells: If further analysis or experimentation of cells is required, a centrifuge can be used to precipitate the cells, discard the supernatant, and collect the cell precipitate.