The pipetting steps of the pipetting workstation

Conclusion first: The core operation process of a liquid transfer workstation includes five major steps: equipment preparation, program setting, calibration verification, operation execution, and subsequent maintenance. Standardized operation can significantly improve the accuracy and experimental flux of liquid transfer.
background
Pipette workstation is a key automated equipment for achieving high-throughput and low error liquid processing, widely used in PCR system construction, ELISA detection, gradient dilution, sample pretreatment and other scenarios. Its operation not only involves hardware settings, but also requires software programming and daily quality control to ensure volume accuracy and repeatability.
For ease of understanding, the following summarizes the core steps into a clear operational framework:
Detailed explanation of operation steps
1. Equipment and consumables preparation
Platform layout: Place the suction head kit, reagent tank, microplate, etc. in the designated area of the workbench (such as ANSI/LAS standard position) according to the programmed position, ensuring that the robotic arm can access them without obstacles.
Consumables matching: Choose compatible suction tips (recommended original or verified brands) to avoid leakage or volume deviation caused by poor sealing.
Power on self-test: After starting the system, perform an empty run test to check whether the movement of each axis is smooth and confirm the closed state of the safety door.
2. Program Setting and Optimization
Create process template: Set the pipetting action in the software by dragging and dropping, such as "suction → transfer → separation → mixing" and other steps.
Parameter configuration:
Set the pipetting volume and velocity (for high viscosity liquids, the flow rate should be reduced);
Enable pre wetting function (suitable for residual liquids);
Enable laser liquid level detection (to prevent air suction or bottoming out)