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From installation to calibration: Operation manual for the entire process of initial use of biosafety sterilizers
Date: 2025-10-21Read: 0
Biosafety sterilizerIt needs to meet stricter biological protection requirements, and the entire process of initial use directly affects the safety and reliability of subsequent use. The following is a detailed step-by-step guide.
Step 1: Prepare the installation environment
Choose an independent and well ventilated room, away from densely populated areas and water sources. The ground should be made of anti-static and corrosion-resistant concrete (thickness>15cm), and a drainage slope (>2%) should be set up to clean up leaks. The installation position should be horizontal (inclination<1 °), away from heat and vibration sources. An operating space of ≥ 80cm should be reserved around the sterilizer (for easy opening and maintenance), and a heat dissipation space of ≥ 50cm should be reserved at the top (to avoid high temperature accumulation). At the same time, connect a dedicated grounding wire (grounding resistance<4 Ω) to ensure leakage protection.
Step 2: Equipment positioning and connection
Use a forklift or crane to place the sterilizer steadily in the installation position and secure it with anchor bolts. Connect the inlet pipe (providing distilled water or deionized water with a water conductivity of<10 μ S/cm to avoid scale blockage of the pipeline) and the drain pipe, ensuring that the interface is sealed (wrapped with raw material tape to prevent water leakage). Connect the power supply and check the circuit protection device. For biosafety sterilizers, it is also necessary to connect high-efficiency air filters to ensure exhaust safety.
Step 3:first timePre operation inspection
Check the inner liner (made of stainless steel material) for scratches or deformation (which may affect sealing), and the door seal (made of silicone material) for completeness (without aging or cracking, otherwise it may cause steam leakage). Inject distilled water into the water tank (when the water level reaches the calibration line, it will trigger dry burn protection if it is too low), clean the protective oil film in the sterilization chamber (wipe it with an anhydrous ethanol cotton ball). Turn on the power, run the no-load self-test program (observe whether the display screen displays parameters such as temperature, pressure, time, and whether each indicator light lights up logically), test the safety valve (manually pull to check flexibility, normal should be able to quickly exhaust and automatically reset).
Step 4: Parameter setting and no-load testing
According to the standards (such as the "Regulations on the Management of Medical Waste" requiring sterilization parameters for infectious waste to be 121 ℃/30 minutes or 134 ℃/10 minutes), input the initial parameters on the control panel (recommended)first timeSelect 121 ℃/30 minutes). Start the empty sterilization program and observe the actual temperature and pressure changes. Check the sound of the equipment during operation (normal is a uniform steam flow sound, without abnormal noises such as metal friction sound), and observe whether there is steam leakage at the door seal. After the no-load test is completed, check if there is any residual condensation water in the inner tank (a small amount of condensation water is normal, but if there is a large amount of water accumulation, it may cause poor drainage).
Step 5: Calibration and Load Testing
Calibrate the sensor using a standard thermometer (accuracy ± 0.5 ℃) and pressure gauge (accuracy ± 0.5kPa) (place the standard probe in the center of the sterilization chamber, compare the displayed value with the actual value, and contact the manufacturer for adjustment if the deviation is>± 1 ℃ or ± 1kPa). During load testing, place simulated infectious waste (such as gauze bags soaked in bacterial culture medium, with specifications consistent with actual use) and run the sterilization program according to standard parameters. After sterilization is completed, take out the sample for biological testing (such as observing the culture medium for 48 hours without bacterial growth to prove sterilization qualification), or use a chemical indicator card (color change meets the standard, such as changing from blue to brown to indicate sterilization conditions). If the calibration or load test fails, it is necessary to repeatedly check the parameter settings, sealing, and sensor status until all indicators meet the requirements.
After completing the entire process operation,Biosafety sterilizerOnly then can it be officially put into use.first timeThe standardized process used is the cornerstone of subsequent safe operation. Only by strictly controlling every step can infectious substances be sterilized and the bottom line of laboratory biosafety be safeguarded.