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Polysaccharide determination
Date: 2025-04-23Read: 2

many sugar determination


Chromatographic conditions:

Chromatographic column:Morphling WD- NH2-2 4.6*250mm 5um

Mobile phase: Acetonitrile-water=75/25(Mixed)

temperature40℃,

detector:IRtemperature35

Current Speed:1.0ml/min

Injection volume:20ul

Samples: Sodium chloride, fructose, glucose, maltose, isomaltose, maltotriose.

chromatographic parameters

Fixed phase: Porous silica gel

Bonding phase: Aminopropyl bonded silica gel

Carbon load: 6%

Specific surface area:320m2/g

Aperture: 120A

Optional particle size: 5um

Chromatogram:

Mixed sample spectrum

image.png

Picture1Sodium chloride


image.png


Picture2: Fructose

image.png

Picture3: Glucose

image.png

Picture4Maltose sugar

image.png

Picture5Isomaltose

image.png

Picture6Malt trisaccharide

image.png






















Conclusion: He Xi BiologicalThe Morphling NH2-2 4.6 * 250mm 5um chromatographic column can achieveThe determination of mixed samples of fructose, glucose, maltose, isomaltose, and maltotriose showed that the separation degree met the requirements and the effect was good.

Ordering information:

Product Specifications Item Number
Morphling WD-NH2-2 4.6*250mm 5um FNH2546250


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