saffronmiddleCrocinAnalysis of

Chromatographic conditions

Chromatographic column:Morphling®WD-C18 4.6*250mm 5um

Mobile phase: Acetonitrile-Water gradient elution

Flow rate: 1.0mL/min

Column temperature: 25 ℃

Detector Model: UV (Ultraviolet Light Detector) (327 nm)

Column parameters

Bonding phase:C18（USP L1）

Particle size:1.8um 、2um、3um、 5µm 、 10µm

Aperture:120, 300

Specific surface area:320m²/g(120 O), 90 m²/g (300O)

Carbon load:17%(120 O), 8% (120 O)

Closing the tail:Double sealed tail

PH stability:1.5-10.0

chromatogram：

test sample

reference standard

Conclusion: He Xi Biology'sWD-C18 4.6 * 250mm 5um chromatographic column can achieve the detection of crocin in saffronSeparation and determination of crocin 1 and crocin 2.

Ordering information: