PTI peptide synthesizerCore principle: Focus on four major categories of problems that beginners can easily handle: operation, parameters, materials, and maintenance. Use simple operations to avoid risks, ensure synthesis efficiency and product purity, and get started quickly without pressure.
1、 Preparation before operation: avoid "basic mistakes"
Be sure to read the operation manual thoroughly, clarify the logic of using the nitrogen power system, reactor, and raw material tank of the PTI synthesizer, and do not blindly press buttons.
Check the installation environment to ensure that the tabletop is stable, dry, and well ventilated. The power supply voltage should match the requirements of the instrument to avoid shaking or abnormal power supply during operation.
Calibrate the instrument in advance, especially after long-term disuse, and test core components such as pumps and sensors through a self diagnostic system without skipping the self-test step.
2、 Material and parameter settings: avoid "configuration deviation"
The resin and protecting group need to be matched. Rink Amide resin is selected for synthesizing amide C-terminal peptides, Wang resin is selected for carboxylic C-terminal peptides, and Fmoc protecting group system is preferred to reduce side reactions.
Amino acids and activators need to be prepared fresh and in proportion. Novices are advised to use pre packaged reagents to avoid manual weighing errors or reagent failure.
The synthesis scale starts from 0.1-0.5mM (suitable for common PTI specifications), and the reaction temperature and time are set according to the preset template. If the Asp sequence is included, the deprotection temperature needs to be lowered to avoid the formation of Aspartimide.
3、 Operation process: Avoid "monitoring blind spots"
Real time monitoring of instrument pressure and leakage. If abnormal pressure occurs, first check if the air path is blocked and do not forcefully continue operation.
Avoid interrupting the program midway. If adjustments are necessary, follow the steps in the manual and do not directly cut off power or break mechanical components.
When synthesizing multiple sequences, ensure that the amino acid sequences corresponding to the three reactors are correct, do not confuse the positions of the raw material tanks, and prevent sequence errors.
4、 Cleaning and Maintenance: Avoid Negligence in Maintenance
Clean immediately after the synthesis is completed, rinse the reaction vessel and pipelines with a specialized solvent to remove residual reagents and avoid corroding the surface of inert materials.
Regularly lubricate the air circuit and connecting components, and do not rinse the inside of the instrument directly with water to prevent short circuits or rust.
When storing, turn off the power of the instrument, remove the reagent and seal it, and place the instrument in a dry instrument cabinet to avoid dust contamination or exposure to humid environments.