QPix^?The microbial cloning screening system adoptsfirst-classThe technology is used to reduce manual or repetitive tasks in the laboratory. It is widely used for rapid, accurate, and efficient screening of gene libraries. According to the Good Manufacturing Practice (GMP) standards, a key requirement for the production of biologics is to ensure sterility to prevent cross contamination. The aim of this study is to demonstrate the sterility of using the QPix 420 system for automatic colony selection and plate laying processes. The system has multiple sterilization functions, including ultraviolet radiation for internal disinfection, needle picking cleaning stations, and halogen lamp drying. These features not only ensure compliance with GMP standards, but also provide economic benefits through the reusability of needle picking. During each picking process, the picking needles undergo multiple cleaning steps in three washing sinksthoroughlyRinse to remove microbial residues, then sterilize by halogen drying.

Advantage:

Method:

Select colonies using the samples, needle types, and disinfection procedures described in Table 1. Each experiment includes the use of consumablesPoison needleInoculate the control well and then use it to eliminatePoison needleInoculate into another well (Figure 1, red well). All target plates (96 well microporous plates) were subjected to bacterial growth analysis after overnight stirring and cultivation at 37 ° C. These data are from validation studies of multiple QPix 420 systems, evaluating the efficiency of QPix disinfection cycles based on the presence of viable biological samples in control wells (Figure 1, green and red wells, respectively).

Result:

In different QPix instruments, a total of 497 control wells were inoculated without cross contamination (Table 2).

Method:

The QPix 400 series can also automatically apply samples onto trays containing agar. The liquid samples in the 96 well microplate can be transferred to the agar surface, allowing for automatic coating of up to 96 samples within 30 minutes. Apply using the parameters listed in Table 3. Each run uses inoculation source wells (Figure 2, green wells) and pure LB medium source wells (Figure 2, yellow wells). Each experiment includes a control area, which is disinfected with needles previously coated with biological samples, and then coated again with LB medium (Figure 2, red for the control area). After overnight cultivation at 37 ° C, analyze the growth of all target plates. Evaluate the efficiency of QPix disinfection cycle by comparing whether there are viable biological samples in the control area.

result

For each QPix instrument, a total of 80 control areas were inoculated, and no cross contamination was detected (Table 4).

Advanced algorithms help to quickly identify and high-throughput select microbial colonies with the desired phenotype. Optimized strain specific needle selection ensures efficient and accurate colony collection, with an efficiency of over 98%. The versatility of the QPix modular system supports the flux expansion of microbiological research, effectively eliminating the risk of cross contamination.