Cisbio's Tag lite HTRF platform can effectively utilize HTRF fluorescence
Mark the protein of interest at the target site for the group. Cell surface receptors can be inserted
Insert SNAP tag plasmid, then transfect cells with this structure and express it
Marked receptors. By adding snap-lumi4 Tb substrate and terbium (Tb)
Acupoint shaped compounds label receptors. For binding experiments, the ligand of the receptor
Label with HTRF receptor fluorophores, such as d2. When d2 ligand is labeled with terbium
When binding to the receptor, a microplate with HTRF function can be used for reading
Time resolved Fluorescence Resonance Energy Transfer (TRFRET) from Receptor to Ligand Detection by Plate Machine