Cisbio's Tag lite HTRF platform can effectively utilize HTRF fluorescence
Mark the protein of interest at the target site for the group. Cell surface receptors can be inserted
Insert SNAP tag plasmid, then transfect cells with this structure and express it
Marked receptors. By adding snap-lumi4 Tb substrate and terbium (Tb)
Acupoint shaped compounds label receptors. For binding experiments, the ligand of the receptor
Label with HTRF receptor fluorophores, such as d2. When d2 ligand is labeled with terbium
When binding to the receptor, a microplate with HTRF function can be used for reading
Time resolved fluorescence resonance energy transfer (TRFRET) from receptor to ligand was detected by a plate machine (Figure 1).
Cisbio provides plasmids encoded with various tags and protein of interest markers to
And frozen cells transfected with these structures. This structure can represent
Proteins that meet the standard, such as receptors, can be labeled with terbium and their receptor ligands
(Excitant or antagonist) labeled with receptor fluorophores. Tag lite platform is suitable for
Used for a wide range of applications, such as receptor dimerization, ligand binding analysis, and secondary signaling
Make an assessment. Combining kinetics can be used to study the structure of drug protein complexes
The rate of fusion and dissociation is a necessary step in optimizing the in vivo efficacy of candidate drugs1.