The 'gold standard' for brucellosis is generally believed to be the isolation and identification of the disease
Originally, Brucella grows very slowly in vitro and usually requires several days of cultivation
It may even take several weeks to produce results, and the experiment requires a biosafety level three certification
The laboratory is staffed by experienced technicians, so this experiment is not conducted
Suitable for on-site instant diagnosis or rapid diagnosis when the epidemic needs to be controlled.
The PCR method has the advantages of fast speed, high sensitivity, and safety
More and more are being applied to the diagnosis of infections in humans and animals, but Brucella
The performance of bacterial PCR detection varies among different laboratories, which affects sample preparation
Important technical aspects such as program preparation, selection of genomic targets, and detection techniques
Standardization has not yet been established[6]Compared with cultivation and PCR methods, in terms of technology
From a perspective, serological diagnostic methods are more economical and relatively simple, making them more suitable
Conduct large-scale screening and diagnosis, especially in epidemic areas. tradition
The serological diagnostic methods also have their own advantages and disadvantages, such as the test tube agglutination test
(SAT)、 Complement binding test (CFT) and other methods are time-consuming, cumbersome, and prone to errors
It is difficult to determine the outcome, and the judgment heavily relies on the experience of technical personnel; tiger
Although the red plate agglutination test (RBT) is easy to operate, it can be obtained within a few minutes
However, its high sensitivity may produce false positives in vaccinated animals
sex[7]False positive reactions can also occur in patients with cross reactive bacterial infections
should[7]Although enzyme-linked immunosorbent assay (ELISA) has high specificity
And sensitivity, but the experimental process requires multiple washes and incubation, which may affect the operator
The professional requirements are high and the steps are time-consuming. Although these shortcomings are significant
Degree can be overcome by combining multiple serological tests, but people
Always seeking a fast, simple, economical, and more accurate way to treat brucellosis
Testing method.