Manual multi-channel pipette (also known as multi-channel pipette) is a commonly used laboratory instrument, widely used for the distribution and transfer of liquids, especially in high-throughput experiments. To ensure accurate operation, avoid errors, and extend the service life of the equipment, the following precautions should be followed when using a manual multi-channel pipette:
1. Proper selection of pipette and pipette
Choose the appropriate capacity: Ensure that the selected pipette matches the volume of liquid to be transferred. For example, the capacity of the pipette should be consistent with the specifications of the pipette used.
Use appropriate pipettes: Different pipettes have different capacity ranges, and compatibility with the pipette should be ensured when selecting them to avoid affecting operational accuracy if the capacity is too small or too large.
2. Check the status of the pipette
Calibrate the pipette: Before use, ensure that the pipette has been correctly calibrated. If the pipette is not calibrated correctly, it may result in errors.
Check the sealing of the pipette: Before use, check if the seal of the pipette is intact to ensure that there is no leakage.
Check the suction and discharge functions of the pipette: Before operation, check if the suction and discharge buttons are functioning properly.
3. Operational skills
Adjust the suction volume: Adjust the suction volume of the pipette according to the volume of liquid that needs to be transferred. If it is a multi-channel pipette, it is necessary to ensure that the suction volume of all channels is consistent.
Vertical operation: The pipette should be kept vertical to avoid tilting, which helps reduce air intake and liquid errors.
Smooth operation: When pressing the suction button, the action should be smooth and fast to avoid liquid splashing or errors caused by improper operation.
Avoid excessive liquid suction: When sucking liquid, do not press the suction button too much to avoid splashing or inaccurate suction.
4. Avoid bubbles
Avoid inhaling bubbles: During the suction process, avoid generating bubbles. Bubbles may cause inaccurate volume readings and affect experimental results.
Eliminate bubbles: After use, ensure that there are no bubbles in the pipette tubing before moving the liquid into the container. If there are bubbles, gently press the drain button to release them.
5. Distribution of liquids
Slow release of liquid: During the pipetting process, a uniform and gentle release speed should be maintained to avoid splashing out due to excessive release.
Synchronized use of multiple pipettes: When using multiple pipettes, ensure that the liquid release from all channels is synchronized and do not allow any one channel to leak.
6. Cleaning and maintenance
Timely cleaning: Clean the pipette immediately after each use, especially after using different types of liquids. Clean with appropriate solvents and cleaning agents to avoid residue interference with the next use.
Avoid drying up: Ensure that all parts of the pipette are dry after cleaning. When storing, it can be placed in a dry place to avoid liquid residue causing spoilage.
Regular maintenance and calibration: Regularly calibrate and maintain the pipette to ensure its continuous good operation. Regular maintenance can be carried out according to the manufacturer's recommendations.
7. Use appropriate liquids
Avoid high viscosity liquids: For liquids with high viscosity or containing particles, avoid using multiple pipettes for transfer, as these liquids may clog the pipetting pipeline and affect operational accuracy.
Temperature effect: The temperature of a liquid may have an impact on its viscosity and volume, ensuring that it is operated at an appropriate temperature.
8. Operating environment
Stable operating environment: Avoid using the pipette in strong airflow, high or low temperature environments. The operating environment should be kept stable to avoid factors such as airflow that may cause liquid evaporation or inhalation of air.
9. Safety protection
Wear appropriate protective equipment: When using a pipette, especially when in contact with toxic, corrosive, or biologically hazardous liquids, gloves, laboratory coats, and protective goggles must be worn.
Avoid direct contact: If hazardous materials need to be transferred during use, ensure that the liquid does not come into direct contact with the skin or eyes to avoid accidents.
summary
When using a manual multi-channel pipette, strictly follow the operating procedures to ensure the selection of appropriate pipettes and liquids, avoiding operational errors or equipment damage. Regular cleaning and maintenance of equipment can also ensure its long-term effective operation and reduce errors in experiments.