Antibiotic microbiological assay is an internationally recognized and classic method for determining antibiotics. Microbial assay can intuitively and specifically reflect the antibacterial activity of antibiotic varieties; Due to the differences in structure and biological activity of different active components, multi-component antibiotics are difficult to accurately characterize the relationship between component composition, content, and biological activity based on chemical determination results; Due to various reasons such as lack of characteristic UV absorption, there is currently no appropriate chemical analysis method to characterize the activity of many antibiotic varieties.