-
E-mail
2224385370@qq.com
-
Phone
13738176644
-
Address
Building B, Champagne Garden, North Software Park, Gongshu District, Hangzhou City, Zhejiang Province
Hangzhou Lebos Experimental Equipment Co., Ltd
Standardizing the cleaning and sealing of replenishment bottles is the key to ensuring the cleanliness and functional integrity of the replenishment system
Date: 2025-11-24Read: 12
Sealed feeding bottles are widely used in biopharmaceuticals, cell culture, fermentation engineering, and laboratory reagent addition scenarios, for continuously and controllably supplementing nutrient solutions, inducers, or buffers in sterile or closed systems. Its structure usually includes a high borosilicate glass bottle body, PTFE/silicone spacer, stainless steel or plastic bottle cap, and sterile interface. Once the cleaning is not done properly, the residue will breed microorganisms, block pipelines, interfere with experimental results, and even damage the entire sterile system. Therefore, establishing a scientific and standardized cleaning process for sealed replenishment bottles is the key to ensuring the cleanliness and functional integrity of the replenishment system.
1、 Instant pre-treatment after use: anti drying and anti fouling
After the replenishment is completed, the residual liquid in the bottle should be immediately rinsed with deionized water or purified water to avoid protein, sugar, or salt from drying up. If containing viscous liquids (such as glycerol or culture medium), soak in an appropriate amount of warm water or low concentration alkaline cleaning agent (such as 1% NaHCO) for 30 minutes to soften the residue.
2、 Disassembly and classification cleaning
Disassemble the bottle cap, spacer, interface and other components, and classify them for processing:
Glass bottle body: Use a laboratory specific cleaning machine for alkaline washing (60-70 ℃, pH10–12）、 Three step process of acid neutralization (pH 4-5) and final washing with ultrapure water; If cleaning by hand, use a long handled soft bristled brush to clean the inner wall, especially the dead corners of the bottle shoulder and bottom;
PTFE/Silicone Spacer: Scratching with hard objects is prohibited. Ultrasonic cleaning (frequency 40kHz, time 10 minutes) can be used in combination with neutral detergent, and then sterilized with high-pressure steam (121 ℃, 20 minutes) to restore elasticity;
Metal/plastic interface: Check if the threads are intact, wipe with non-woven fabric dipped in ethanol to ensure no particle residue.
3、 Final rinsing and drying
All components must be rinsed at least three times with ultrapure water of ≥ 18.2M Ω· cm to remove ions and residual cleaning agents. Then place it in a clean oven at 60-80 ℃ and dry for 2 hours, or use oil-free clean compressed air to blow dry. It is strictly prohibited to air dry naturally to prevent the deposition of particles or the formation of water stains in the air.
4、 Integrity check and aseptic storage
Visual inspection after cleaning: The glass has no cracks, the spacer has no aging cracks, and the sealing surface has no scratches. Ensure that the spacer is flat and embedded during assembly, and tighten the bottle cap in place. After sterilization, store in a Class 100 clean cabinet or double layered sterile bag, labeled with the cleaning date and expiration date (recommended ≤ 7 days).