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Remember! Scientific cleaning of wide mouth reagent bottles is the foundation for ensuring the purity of experiments
Date: 2025-09-15Read: 14
As a commonly used container for storing solid reagents, standard samples, or culture media in laboratories, the cleanliness of wide mouth reagent bottles directly affects the accuracy of experimental results and the stability of samples. Residual chemicals, microorganisms, or cleaning agents may cause cross contamination, catalytic side reactions, or affect pH values. Therefore, mastering the scientific and systematic cleaning methods for wide mouth reagent bottles is the foundation for ensuring the purity of experiments.
Step 1: Initial dumping and residual liquid treatment
Immediately pour out the remaining reagents after use to avoid drying and clumping. For toxic, corrosive, or organic solvent residues, they should be classified and collected according to laboratory hazardous waste regulations, and it is strictly prohibited to directly pour them into the sewer. Solid residues can be gently scraped off with a clean scraper to avoid scratching the bottle wall.
Step 2: Rinse with clean water and pre wash with soft brush
Rinse the inner wall of the bottle repeatedly with tap water to remove soluble impurities. Choose a bottle brush (made of nylon or sponge material) that matches the bottle mouth, and gently brush the inner wall, bottom, and threaded mouth with water. Avoid using metal brushes or hard bristles to prevent scratches and become a breeding ground for bacteria. The wide mouth design facilitates the brush head to penetrate deeply, ensuring cleanliness.
Step 3: Deep treatment with specialized cleaning agents
Choose cleaning solution based on the properties of the residue:
Inorganic salts: Soak in 5-10% dilute nitric acid or dilute hydrochloric acid for 1-2 hours to dissolve metal ions;
Organic matter or oil stains: Soak in laboratory specific detergents, alkaline cleaning solutions (such as NaOH ethanol solution), or organic solvents (acetone, ethanol);
Microbial contamination: Soak and disinfect with 0.1% sodium hypochlorite solution or 75% alcohol.
After soaking, rinse again with a bottle brush to ensure that the stains are removed.
Step 4: Pure water final wash and deionized water rinse
After pouring out the cleaning solution, rinse it with tap water for 3 – 5 times until there is no foam or greasy feeling. Rinse 2-3 times with deionized water or ultrapure water, shake every 100mL of water in the bottle for 30 seconds, and then pour out to remove residual ions and cleaning agents. For trace analysis experiments, it is recommended to rinse at least 3 times to ensure that the conductivity meets the standard.
Step 5: Drying and Storage
After cleaning, place the bottle upside down on a clean drying rack and let it air dry naturally. If rapid drying is required, it can be placed in a 60-80 ℃ oven for drying, but high temperature should be avoided from causing stress rupture of the glass. Do not use cloth or tissue to wipe the inner wall to prevent fiber residue. Immediately cover with a clean bottle cap (or sealing film) after drying and store in a dry, dust-free reagent cabinet to avoid secondary contamination.