1、 Daily cleaning and disinfection

1. Cleaning of the surface of the box
   • Wipe the outside of the box with a soft damp cloth daily, avoiding the use of corrosive cleaning agents (such as alcohol concentration>75%).
   • Clean the sealing strip of the box door with a neutral cleaner (such as dishwashing detergent) every week to prevent dust accumulation and air leakage.
2. Internal cavity cleaning and disinfection
   • After the training is completedImmediately remove the culture and wipe the inner walls, shelves, and shaking trays with 75% alcohol to prevent residual microorganisms from breeding.
   • Regular deep disinfectionUse a UV lamp to irradiate the inner cavity for 30 minutes every month, or spray 0.1% chlorhexidine solution and ventilate and dry.
   • Stubborn stain treatmentFor protein stains, soak them in a 2% sodium bicarbonate solution and wipe them off; For fungal contamination, it is necessary to thoroughly clean and spray 75% alcohol and expose to ultraviolet radiation for 1 hour.

2、 Key component maintenance

1. Calibration of Carbon Dioxide Sensor
   • Calibrate the sensor with standard gas (such as 5% CO ₂) every month to ensure accurate concentration display. If the deviation is greater than 0.5%, contact the manufacturer for adjustment.
   • Avoid contact with high concentrations of alcohol or organic solvents to prevent damage to the sensor.
2. Temperature and humidity system inspection
   • Verify the temperature inside the box with a calibrated thermometer every week. If the deviation is greater than ± 0.5 ℃, adjust the PID parameters or replace the heating element.
   • Check the water level of the humidification tray to ensure stable humidity (usually maintained at 95% RH ± 3%). If the humidifier malfunctions, it is necessary to replace the distilled water or clean the scale.
3. Maintenance of oscillation system
   • Check the tightness of the oscillating motor belt every month. If it is too loose, it will cause unstable amplitude, and if it is too tight, it will accelerate bearing wear.
   • Clean the oscillation tray bearings and apply specialized lubricating grease (such as silicon-based grease) to reduce friction noise.

3、 Environment and operating standards

1. Requirements for placement environment
   • The equipment should be kept away from heat sources, direct sunlight, and ventilation openings. The ambient temperature should be controlled at 18-25 ℃ and the humidity should be less than 70% RH.
   • Reserve at least 10cm of heat dissipation space to avoid stacking debris that may affect ventilation.
2. Operation precautions
   • Open the box for no more than 1 minute to reduce temperature and humidity fluctuations.
   • Avoid frequent starting and stopping of equipment, and it is recommended to run for more than 8 hours per day to stabilize the system.
   • Regularly check the integrity of the door seal, and replace it promptly if it ages or cracks to prevent external contamination from entering.

4、 Long term disuse maintenance

• After cleaning the interior, keep the door slightly open (about 5cm) for ventilation to prevent mold growth.
• Disconnect the power, cover with a dust cover, and store in a dry environment.
• Before reactivation, it is necessary to run without load for 24 hours, verify that all parameters are normal, and then put it into use.

promptIt is recommended to establish a maintenance record table to record the cleaning, calibration, and repair time for easy tracking of equipment status. If there is a malfunction (such as frequent alarm, temperature and humidity loss of control), the machine should be stopped immediately and professional engineers should be contacted for repair. Do not disassemble it by yourself.