dislikeOxygen cultivationThe box isforcultivateanaerobicThe key to microorganismsEquipment,During operationStrict compliance is requiredThe following precautionsTo ensure the experimentSafety andCultivation effect：
1Before operationprepare
1.checkqisource
confirmnitrogenQihydrogenCarbon dioxideqiBottle pressure充足（usuallyneed≥0.3MPa），gaspurity≥99.99%To avoidimpurityinfluencedislikeOxygen environment.
Check the gaspipelineIs there a connectionseal, noneLeakage（Available soapwater testing）.
2. Pre restorationculture medium
culture mediumusePrevious requirementsboildriveOxygen,andQuickly put it inanaerobicInside the boxpre-Restore 24More than hours(such asusebladeazureinstructionAgent,Ensure colorlessstate）.
3. equipmentpre-run
in advanceActivate disgustOxygen tankpower supply，Run at least2 hours（orpressInstruction manual requirements）Ensure thatInside the boxReaching disgustOxygen state(Oxygen)Concentration<1ppm, availableoxygenindicatorverify）.

IIoperating processPrecautions
1. sampletransmitspecification
use dedicatedTransfer cabin(Transition)Cabin):
First drawVacuum（to 0.1MPa），RechargenitrogenQi（Repeat 2 3Secondly,ensureoxygenReplaced.
avoidfrequentopenTransfer cabinEvery timeoperationLater neededrecoveranaerobicenvironment
2.individualprotection
Wearing withoutpowdergloveAvoid powderPollution boxinternal environment）If necessaryUse a maskandgogglesMirror.
operationgas(such asHydrogen gas)Strictly prohibitedbrightFire orstatic electricity.
3.boxInternal operationkey points
toolandItems requiredUsing alcoholDisinfect anddryAfter doing itPut it in,Avoid bringing inoxygenOr moisture.
actionlightSlow down,Reduce airflowDisturbance,avoidglovedamaged（regularlyexamination glovessealSex).
cultivatedish、test tubeWaiting for containerSealed is required（If usedanaerobicBag orglueblock）.

IIIusePost maintenance
1. Cleaning anddisinfect
定期用75%Alcohol orSpecialized disinfectionWipe with detergentInside the boxThe wallgloveAnd tray，防止microorganismPollution.
wasteLiquidWaste culture mediumneedTimely relocationOut,Avoid breedingmiscellaneousBacteria.
2.gasmanagement
Turn off the gasbottlevalveafter, need toRelease pipelineresidualPressure（防止逆Flow).
long-term unusedWhen,at least weeklyboot upOncewithMaintain disgustOxygen environment.
3. Catalyst regeneration
Palladium catalyst(ExceptOxygen agent）Every2 3Month in160℃bake2 hoursRegeneration,failureaftertimelyreplace

four, Safety警示
1. Hydrogen risk
hydrogenYi RExplosive，ensureboxgroundingGood,operationStay away from timespark、high temperatureequipment
2.hypoxiarisk
avoidMove the headstretchEntering the boxinside， 防止Suffocation（boxInternally fornoneOxygen environment）.
3. emergency response
asEncountering leakageQi oron fireImmediatelyTurn off the gasSource,Using carbon dioxidefire extinguisher(Not allowed)Using water ordryPink).

fiveCommonProblem Handling
boxInternal humidityhigh：addsiliconGel dryingAgent oropendehumidifyFunction.
oxygenElevated concentration：examination glovessealing performanceTransferCabin operationstepwhetherstandard,Or replace itcatalyzer.
cultivatepollution：troubleshoottransmitCabin disinfectionprogramCulture mediumIs sterilization effectiveBottom.