The following is a summary of the maintenance manual for a three gas incubator (usually referring to cell culture equipment that controls O ₂, CO ₂, and N ₂ concentrations) for reference:
Maintenance Manual for Three Gas Incubator
# 1. routine maintenance
Cleaning and disinfection
Wipe the inner cavity, shelves, and door seals with sterile gauze dipped in 75% alcohol or specialized disinfectant every week (avoid corrosive solvents).
Clean the water tank (if equipped) monthly to prevent microbial growth, using distilled or deionized water.
humidity control
Check the water level and replenish sterile water in a timely manner to avoid dry burning.
When not in use for a long time, drain the water tank and keep it dry.
Gas monitoring
Record the concentration values of O ₂ and CO ₂ daily and confirm that they are consistent with the set values (it is recommended to use an external calibrator for regular calibration).
# 2. Regular maintenance (monthly/quarterly)
Filter replacement
The intake HEPA filter needs to be replaced every 6 to 12 months (depending on usage frequency) to reduce the cycle of environmental pollution.
Check if the gas pipeline is leaking (at the soap water detection joint).
sensor calibration
The CO ₂ sensor is calibrated every 6 months using standard gas or an infrared calibrator.
O ₂ sensors are recommended to be calibrated annually by manufacturers or professional technicians.
Door seal inspection
Check whether the sealing strip is aged or cracked to avoid gas leakage that affects gas stability.
# 3. Long term discontinuation and activation
Before discontinuation
Clean the inner cavity, empty the water tank, turn off the air source, and turn off the power.
Keep the door slightly open for ventilation to prevent odors or mold.
reactivate
Before starting up, check the circuit and air circuit, run without load for 24 hours, and calibrate the parameters.
# 4. Common fault handling
Unstable concentration of CO ₂
Check if the cylinder pressure and pressure reducing valve are normal, or if the sensor needs calibration.
Abnormal humidity
Clean the humidity sensor and check the water supply system of the water tank.
Alarm Alert
Record the error code and refer to the instruction manual for troubleshooting; Contact after-sales service to handle circuit or sensor faults.
# 5. Safety precautions
Gas safety
Ensure that the gas cylinder is fixed and kept away from sources of fire; Excessive N ₂ may cause suffocation, and good ventilation is required during operation.
biosafety
Disinfection is required after handling contaminated samples to avoid cross contamination.
# 6. Recommended spare parts list
HEPA filter, door seal, humidity sensor, CO ₂ infrared sensor (matched according to model).