Real time fluorescence quantitative PCR instrumentIt is an instrument used for fluorescence measurement, which adopts a side fluorescence collection method. Six fluorescence channels are collected simultaneously, and 96 samples and six fluorescence channels can be detected within 5 seconds, greatly saving detection time. This product has powerful software and hardware functions to meet the needs of customers in multiple scenarios.
Product Features:
Strong fluorescence signal, low background noise, and high sensitivity.
Complete the detection of all fluorescence channels for 96 samples within 5 seconds.
Adopting LED light source design, energy-saving and environmentally friendly, with long service life and maintenance free.
The automatic unloading function can be used in conjunction with automated workstations, resulting in high work efficiency.
PC operation, one computer can control multiple instruments and run multiple experiments at any time.
The software analysis function is powerful and can perform qualitative/quantitative analysis HRM、 Genotyping, etc.
Preparation before the experiment:
Consumable selection: Choose the appropriate eight tube, 96 well plate or 384 well plate according to the instrument model, and confirm the instrument configuration in advance.
Reagent preparation: Place qPCR premix, template DNA, primers, enzyme free water and other reagents on an ice box and place them in a biosafety cabinet.
Instrument inspection: After turning on the device, enter the password and wait for the self-test to complete (the indicator light turns green) before starting the software.
Reaction system configuration:
Mixed reagents: Calculate the dosage according to the instructions, add the pre mixed solution, enzyme free water, and primer into the Ep tube, invert and mix well.
Sample addition: Add the mixed solution to the left two columns of the eight connected tube, and add the template to the right. Set two parallel samples for each concentration.
Centrifuge: Immediately centrifuge after closing the tube cap to ensure uniform mixing of the solution.
Instrument operation:
Loading samples: Remove the instrument tray and place the eight tube or 96 well plate (adapter is required for the eight tube), paying attention to symmetrical and balanced placement.
Set up program:
Select the type of experiment (such as SYBR Green).
Set temperature program (such as pre denaturation at 95 ℃ for 30s, amplification stage at 95 ℃ for 5s, 60 ℃ for 30s, cycling 40 times).
Confirm the hot lid temperature and reaction system volume (e.g. 20 μ L).
Start running: After saving the program, start the experiment without pulling the reaction chamber during the process.
Result analysis:
Data collection: After the program ends, amplification curves and melting curves are automatically generated. It is necessary to check whether the curves are smooth and whether the results of the wells are uniform.
Threshold adjustment: If it is a qualitative experiment (such as virus detection), negative/positive controls need to be set and the threshold manually adjusted to improve accuracy.