The Hamilton dilution instrument adopts micro injection syringe technology and active displacement liquid suction and discharge method, which can achieve high processing accuracy and precision, greatly avoiding human errors between different operators and ensuring the reliability of experimental results. The operable volume range is wide, suitable for various experimental needs of different scales. Whether it is a small amount of scientific research samples or large-scale industrial production, it can be easily handled.
Compared to traditional manual dilution methods, Hamilton diluents can improve work efficiency. It can automatically complete a series of complex dilution operations according to preset programs, without the need for long hours of manual duty and frequent operations. This not only saves labor costs, but also enables more sample processing tasks to be completed within a unit of time. Automated operations reduce the interference of human factors and lower the risk of sample contamination. At the same time, the internal design of the instrument also takes into account the ease of cleaning, making it convenient for users to maintain the hygiene of the equipment during use. The entire process is not affected by solution viscosity, vapor pressure, and temperature, ensuring the stability and consistency of the experiment. This is particularly important for scientific research that requires strict control of experimental conditions.
The measurement steps of Hamilton dilution instrument:
1. Preparation work
-Choose the appropriate instrument model and parameter settings: Based on the experimental requirements, select a Hamilton dilution instrument with the corresponding measurement range and volume error that meets the requirements. At the same time, determine appropriate accessories and operating modes based on the properties of the liquid to be processed, such as viscosity, corrosiveness, etc.
-Cleaning and disinfection: Wipe the instrument casing, operation panel, and platform with ultrapure water to remove dust and debris; For components that come into contact with liquids, such as liquid tanks, suction tubes, etc., soak or wipe them with appropriate disinfectant (such as 75% ethanol), and then rinse them clean with ultrapure water to prevent cross contamination.
-Calibration instrument: Use standard solution for comparative measurement and adjust the instrument based on actual readings to ensure its accuracy. This is a crucial step in ensuring the reliability of subsequent experimental results.
2. Sample loading and dilution operation
-Placement of containers: Place appropriate containers for holding the original solution and diluted solution in specific locations on the instrument, ensuring that the containers are stable and well sealed to avoid liquid leakage or volatilization affecting concentration.
-Inhalation of original solution: Through the control program or manual operation of the instrument, the pipette is accurately immersed into the original solution, and the suction function is activated to accurately suck a certain amount of original solution according to the preset volume. During this process, it is important to observe whether the liquid absorption process is smooth and whether there are any bubbles generated.
-Add diluent: After filling the original solution, transfer the pipette to the container containing diluent, activate the corresponding function again, and add diluent according to the set ratio. Some models may support automatic mixing function. If not, gently shake or stir the container to mix the solution thoroughly and evenly.
3. Allocation and Collection
-Set the packaging parameters: According to the experimental requirements, set the packaging parameters for the liquid volume that each target container should receive on the instrument.
-Perform packaging task: After confirming that the parameters are correct, start the packaging operation and accurately distribute the diluted solution to each target container. During the packaging process, it is important to closely monitor the operational status of the instrument to ensure the accuracy and stability of the packaging.
4. Data recording and processing
-Record relevant information: Timely record the key information of each dilution operation, including the original solution concentration, dilution ratio, instrument number used, operator name, and operation time, for subsequent traceability and analysis of data.
-Data analysis and validation: Conduct necessary testing and analysis on the diluted solution obtained, such as using spectrometers, chromatographs, and other equipment to determine its concentration and verify whether the dilution effect meets expectations. If significant deviations are found, the cause should be identified and readjusted.