1. The installation and disassembly of chromatography columns must be carried out at room temperature.
2. The filling column has card sleeve sealing and gasket sealing. There are three types of card sleeves: metal card sleeve, plastic card sleeve, and graphite card sleeve. They are not easy to tighten too tightly during installation. The gasket type seal needs to be replaced with a new gasket every time the chromatographic column is installed (Shimadzu chromatography uses gasket sealing).
3. Are the two ends of the chromatographic column plugged with glass wool. Prevent glass wool and fillers from being blown into the detector by the carrier gas.
The length of insertion for the installation of capillary chromatography columns should be determined according to the instructions of the instrument. Different chromatographic vaporization chamber structures require different insertion lengths. It should be noted that if you use a capillary column without splitting and a packed column interface for the vaporization chamber, the capillary column connected to the vaporization chamber should not penetrate too much, just slightly beyond the sleeve. The influence of the ratio of hydrogen to air on the FID detector. The ratio of hydrogen to air should be 1:10. When the hydrogen ratio is too high, the sensitivity of the FID detector drops sharply. When using chromatography under other conditions, if the sensitivity drops, the flow rate of hydrogen and air should be checked. Hydrogen and air have a type of gas that makes a "bang" sound when ignited, and then extinguishes the fire. Generally, when you ignite with electricity, it extinguishes. If you ignite again and then extinguish again, it is due to insufficient hydrogen gas.