96 hole positive pressure extraction deviceIt is a high-throughput sample pretreatment device widely used in fields such as life sciences, molecular biology, drug development, and clinical testing. It is mainly used for rapid and efficient extraction and purification of biomolecules such as nucleic acids, proteins, and metabolites. This device is based on the principle of positive pressure drive, which applies controllable positive pressure gas (usually clean compressed air or nitrogen) to the upper part of a 96 well deep hole plate or filter plate to uniformly and synchronously push the liquid sample through the packing in the solid phase extraction column (SPE column) or centrifuge column, thereby achieving the enrichment, purification or desalination of the target component.
This device typically consists of a pressure control system, gas distribution module, adapter bracket, and operating interface. It is compatible with standard 96 well microplate specifications and supports batch processing of up to 96 samples, significantly improving experimental efficiency and reducing human error. Its core advantage lies in the adjustable pressure (generally ranging from 0-15 psi or higher), ensuring that samples with different viscosities or complex matrices can obtain stable flow rates, avoiding the problems of time-consuming and uneven flow rates in traditional gravity methods, and overcoming the disadvantages of strong equipment dependence and limited flux in centrifugal methods.
96 hole positive pressure extraction deviceThe installation method is as follows:
1. Prepare environment and equipment: Place the device on a flat and stable experimental platform, avoiding direct sunlight or proximity to heat sources. Ensure that the operating environment meets the equipment requirements (temperature 10 ℃~40 ℃, humidity 10%~90%). Check if the pressure of the gas source is within the range of 0.35-0.7Mpa, and confirm that the gas source has been filtered and dehydrated to prevent impurities or moisture from entering the equipment.
2. Install consumable components:
Choose a suitable 96 well plate: Choose a solid-phase extraction plate (SPE), solid-phase support liquid-liquid extraction plate (SLE), or protein precipitation filtration plate (PPT) according to experimental requirements to ensure compatibility between the plate specifications and the equipment. Some models require special specification orifice plates to be installed through adapters.
Installation of collection plate: Place 1mL or 2mL collection plate in the designated position of the equipment, ensuring alignment with the extraction plate to avoid liquid leakage.
Check sealing: Confirm that the extraction plate and collection plate are installed in place, and check whether the sealing gasket is aging or deformed. If air leakage is found, the sealing gasket needs to be reinstalled or replaced.
3. Connect the gas source and equipment:
Connect the external air source to the device's air inlet through a gas pipe, ensuring that the connection is secure and not loose.
Open the gas source valve and adjust the pressure to the desired range for the experiment (0-15psi in the low-pressure zone, 0-95psi or higher in the high-pressure zone). Some models are equipped with gas filtration pressure regulating valves and require regular replacement of filter elements (such as 4 μ m filter elements).
4. Debugging and Calibration:
Pressure test: Start the equipment, observe whether the pressure gauge reading is stable, and check whether the pressure at each hole position is uniform (uniformity ≤± 10%). If the pressure is abnormal, it is necessary to check the air circuit connection or the setting of the regulating valve.
Flow rate verification: By processing standard samples, test the consistency of flow rate between holes. If the flow rate fluctuates by more than ± 5%, it is necessary to check the flatness of the pressure plate (height error ≤± 0.1mm) or the blockage of the air path.
Safety function check: Test the anti pinch design, overvoltage protection and other safety functions of the equipment to ensure safe operation.
5. Preparation before operation:
Sample processing: Use a pipette to evenly add the sample into each well of the extraction plate to avoid the formation of bubbles. If the viscosity of the sample is high, the equipment pressure can be pre adjusted to a higher range (such as 80-100psi).
Program settings: Set the extraction program according to experimental requirements (such as parameters such as pressure, time, flow rate, etc.). Some models support preset programs that can be directly called.
6. Start the extraction program:
Press the start button, and the device will automatically complete steps such as pressurization, extraction, and elution. Avoid touching the air plate or 96 well plate during operation to prevent accidents.
Observe the operation status of the equipment to ensure that there are no abnormal situations such as air leakage or blockage. If there is an alarm or error prompt, stop the machine immediately for inspection.
7. Complete extraction and cleaning:
After extraction is complete, close the gas source valve and lower the gas plate to its initial position.
Remove the extraction plate and collection plate, and dispose of the waste. Clean the inside and outside of the equipment with deionized water or specialized solvents to avoid residual samples corroding the components.
Regularly clean the air interface and pressure plate surface to prevent dust or impurities from accumulating.