NikonTS2RSolution for Replacing Fluorescent Light Source in Inverted Fluorescence Microscope

——with innovationLEDTechnology as the core, reducing maintenance costs and usage barriers

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NikonTS2RInverted microscopes are widely used inCell biology, drug screening, live cell imagingDue to its high compatibility and stability, it has become a core equipment in laboratories. However, its high-frequency usage scenarios (such as long-term dynamic observation and multi-color fluorescent labeling experiments) require high load requirements for fluorescent light sources, and the limitations of traditional light sources further exacerbate equipment maintenance pressure.

1、 NikonTS2RLimitations of original fluorescent light sources

NikonTS2RThere are two ways to connect the fluorescent light source of an inverted microscope:

1. Body integrationLEDlight sourceLocated on the back of the microscope, supporting four independent channelsLEDIlluminator, with a long lifespan (approximately)20,000The advantages of being available in hours and ready to use, but with higher procurement costs.

2. External liquid core light pipe light sourceConnect mercury lamps or metal halide lamps through ports (such asNikon intensitySeries). This type of light source has a lower initial procurement cost, but there are significant drawbacks:

o mercury lampThe lifespan is only about200At an hour, frequent replacement of light bulbs is required, resulting in high maintenance costs and a risk of mercury pollution.

o metal halide lamp: Approximately lifespan2000Although the cost is better than mercury lamps in terms of hours, it is still much lower than mercury lampsLEDLight source, and preheating time is required.

For users with limited budgets, external mercury lamps or metal halide lamps are often chosen“Low cost solution”However, frequent replacement of light bulbs and maintenance shutdowns during long-term use actually increase overall costs, especially in high-frequency fluorescence observation scenarios where the disadvantages are obvious

3. Typical manifestations of performance degradation of traditional light sources (mercury lamps or metal halide lamps):

• The fluorescence excitation intensity significantly decreases, the imaging signal-to-noise ratio decreases, and even the target fluorescent dye cannot be excited.

• The fluctuation of light source brightness leads to inconsistent image contrast in the same experiment.

• Frequent triggering of microscope alarm prompts“Light source malfunction”The experiment needs to be interrupted to investigate the problem.

4. The dilemma of repairing and replacing traditional light sources from the original factory

• Shutdown riskNikon's older models of mercury lamps/The metal halide lamp light source has gradually ceased production, and the supply of spare parts is unstable.

• High cost and long cycleThe cost of replacing the original light source is high (the cost of a single replacement can reach tens of thousands of yuan), and the delivery cycle of imported accessories is several months, which seriously affects the progress of the experiment.

• Compatibility limitationsIf old models of microscopes are forcibly upgraded to the new generation from the original factoryLEDModules may face issues with structural modifications or mismatched control protocols.

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2、 Alternative solution core: New typeLEDTechnological advantages of fluorescent light sources

Regarding the pain points of original light sources, toinnovationLEDTechnology as the coreFluorescent light sources have become a better choice, with advantages including:

1. ultra-long lifespan：LEDThe lifespan of the light source can reach20,000For hours or more, it is mercury lamp100Double, metal halide lamps10Significantly reducing replacement frequency and maintenance costs.

2. Ready-to-useNo preheating required, maximum brightness can be achieved upon power on, improving experimental efficiency.

3. Strong stability：LEDThe light source has stable intensity, avoiding the fluorescence intensity attenuation caused by aging of traditional light bulbs.

4. Environmental SafetyDoes not contain harmful substances such as mercury and complies with laboratory safety regulations.

5. Multispectral coverageBy combining different wavelengthsLEDModule, flexible adaptation to common fluorescent dyes (such asDAPITheFITCTheTRITCEtc.).

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3、 Wright OptoelectronicsXLED-400Specially designed for NikonTS2RoptimizedLEDSolution

To address issues such as aging of traditional light sources and discontinuation of original accessories, third-party high-performance solutions are adoptedLEDFluorescent light sources can fundamentally avoid dependence on the original factory supply chain, while significantly reducing the total lifecycle cost.Guangzhou Laite Optoelectronic Technology Co., LtdLaunchedXLED-400microscopeLEDFluorescent light sourceSpecially designed to be compatible with NikonTS2RDesign an inverted microscope to provide a cost-effective alternative to fluorescent light sources.

Core features of the product:

1. compatible design：LEDThe light source is designed with open compatibility, eliminating the need for original factory accessories and solving the problem of outdated equipment“No light replaceable”The difficult problem.

2. Seamless adaptation: Directly connected through liquid core light pipe interfaceTS2RMicroscope, no need to modify the original structure.

3. Wide spectral output: Support6-8通道LEDCombination (optional UV)/Visible light band), covering350-750nmScope, meeting the requirements of multi-color fluorescent labeling.

   

4. intelligent controlEquipped with a touch screen or software control module, it can accurately adjust the light intensity and quickly switch the excitation wavelength.

5. ultra-low energy consumptionPower consumption is only comparable to that of traditional light sources30%Long term use saves electricity costs.

6. Fast deliveryWright OptoelectronicsXLED-400Long term stable supply of third-party products, supporting fast delivery (usually1-2Arrived within the week)

7. Free trial serviceWright Optoelectronics provides prototype testing and supports users to verify the results on site before making decisions.

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4XLED-400 LEDMaterial composition and access scheme of fluorescent light source

1. Material composition: modular design, flexible adaptation

XLED-400 LEDFluorescent light sourceAdopting a modular architecture, the core components include:

• LEDLight source hostIntegrated high powerLEDModule (optional UV)/Visible light band, heat dissipation system, and driving circuit provide stable excitation light output.

• Liquid core light pipe adapterSpecially designed for NikonTS2RCustomization of optical conduit interface to ensure efficient coupling of optical paths95%To avoid loss of light energy.

• Intelligent control box: Supports touch screen orPCEnd software control, programmable to adjust light intensity and wavelength switching (supports multi-channel timing control).

• Power supply and cablesGlobal universal voltage input（100-240V）Equipped with electromagnetic interference shielding wires to ensure compatibility of laboratory equipment.

• Optional accessories：

• Multi wavelengthLEDModules (such as385nmThe470nmThe555nmThe635nmEtc.), freely combine according to experimental requirements.

• External trigger interface, compatible with synchronous signals.

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2. Connect to NikonTS2RSteps of microscope

XLED-400passLiquid core optical conduit interfaceDirect compatibilityTS2RThe specific connection process for the microscope is as follows:

1. Remove the original external light sourceTurn off the power of the microscope and disconnect the light pipe connection of the original mercury lamp or metal halide lamp light source.

2. Install the light pipe adapter：

• Translate into EnglishXLED-400Inserting the matching liquid core light pipe adapterTS2RmicroscopicExternal light source port(Original light pipe interface), tighten the fixed lock buckle.

• Connect the adapter with Nikon standard light guide (or Wright custom light guide)LEDLight source host.

3. Connection between power supply and control system：

• Translate into EnglishLEDConnect the host to the power supply and connect the control box to the microscope computer (optional for software linkage control).

4. Calibration and Verification：

• After turning on, select the target excitation wavelength through the touch screen of the control box (such asDAPIcorrespond to385nm）Adjust the angle of the light guide to achieve uniform distribution of fluorescence within the microscope field of view.

• Verify the excitation efficiency using standard fluorescent samples (such as fluorescent microspheres) and compare the performance of the original light source.

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3. Technical compatibility statement

• Optical path matching：XLED-400The spectral output range（350-750nm）Covering NikonTS2RMost of the filter systems do not require replacement of the original dichroic mirror or emission filter.

• control protocol: SupportTTLTrigger signal.

• physical spaceThe host has a compact size and can be placed next to a microscope or in a cabinet, saving laboratory space.

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5、 Why choose a third partyLEDLight source instead of original accessories?

1. cost-effectiveness: Original packagingLEDThe module is expensive, andXLED-400Cost reduction under equivalent performance50%The above.

2. Compatibility with old devicesReplacing old microscopes with original factory light sources has low cost-effectiveness, and third-party solutions are more suitable for long-term use needs.

3. Flexible upgradeUsers can choose according to their needsLEDBand, avoiding the limitations of fixed factory configurations.

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6、 Summary of program advantages

passStandardized interfaceandmodular design，XLED-400Achieving compatibility with traditional microscopes at extremely low modification costs:

1. No hardware modification requiredRetain the original structure of the microscope to avoid the risk of equipment depreciation caused by modifications.

2. Plug and Play：10Installation can be completed within minutes, and laboratory personnel can operate independently.

3. Continuous upgrade capabilitySubsequently, it can be replacedLEDExtend the wavelength range of the module (such as near-infrared band) and adapt to emerging fluorescent probes.

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7、 Obtain solutions and technical support

For NikonTS2RUsers,XLED-400 LEDFluorescent light sourceThrough technological innovation, it has been achieved“Low cost investment+High performance output”Balance, especially suitable for laboratories with limited budgets but pursuing efficiency and stability.

Take immediate action：

• Contact Wright Optoelectronics for assistanceFree trial prototype, ExperienceLEDExcellent performance of the light source.

· Visit or call customer service to obtain customized spectral configuration solutions.

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Through a clear bill of materials and a simple access process,XLED-400For NikonTS2RThe user providedZero threshold upgrade planTo solve the pain points of short lifespan, difficult maintenance, and production downtime risks of traditional light sources.

By replacing traditional mercury lamps/Metal halide lamp scheme,XLED-400For NikonTS2RUsers have provided sustainable fluorescence imaging solutions to help laboratories reduce costs and increase efficiency.