Instructions for use

1. Power on

Turn on the power switch on the back of the instrument, and the instrument will display a power on screen. Follow the prompts on the screen to calibrate the instrument zero point before conducting the test (after turning on, press the "zero" button on the test interface to zero all channels simultaneously). The instrument will enter standby testing mode.

2. Reagent preparation

2.1 Buffer solution: Take 1 packet of buffer solution and add it to 500mL distilled water or purified water, stir and dissolve to prepare phosphate buffer solution (pH 7.6), and store at room temperature.

2.2. Color developer: Dissolve 1 bottle of color developer in 25mL of buffer solution. When using, take 100 μ L and store in a refrigerator at 4 ℃.

2.3 Substrate: Dissolve 1 bottle of substrate in 12.5mL distilled water or purified water, take 100 μ L and store in a refrigerator at 4 ℃ during use; Or take 1 bottle of substrate and dissolve it in 2.5 mL of distilled or purified water. When using, take 20 μ L and store it in a refrigerator at 4 ℃.

2.4 Acetylcholinesterase: Enzyme preparations do not require preparation and can be taken directly. When using, take 100 μ L and store it in a refrigerator at 4 ℃.

3. Sample extraction

Take 2g of fruit and vegetable samples (4g for tubers), cut leafy vegetables into fragments of about 25px square, take cross-sectional samples of tubers or their epidermis, place them in a triangular flask, add 10mL of buffer solution, shake for 1-2 minutes, pour out the extraction solution, let it stand for 2 minutes, and wait for testing. If the extraction solution is cloudy or contains too many impurities, it can be filtered before retesting.

test

1. Control test: Add 2.5mL of buffer solution to the reaction bottle, then add 100 μ L of enzyme solution and color reagent separately, mix well, let the reaction stand for 10 minutes, add 100 μ L (or 20 μ L) of substrate, shake well and immediately pour into the colorimetric cup, and promptly place it into the measuring chamber channel of the instrument. Press the 'Comparison' button, the display screen will delay the set number of seconds, and the measurement time will start. After the timer is completed, the display screen will show the increment of the reference absorbance (ΔΑ) and prompt completion. When conducting control testing, other channels can also conduct sample testing simultaneously.

2. Sample testing: Add 2.5mL of the test solution to the reaction bottle, then add 100 μ L of enzyme solution and color reagent separately, mix well, let the reaction stand for 10 minutes, add 100 μ L (or 20 μ L) of substrate, shake well and immediately pour into the colorimetric dish, and promptly place it into the measuring chamber channel of the instrument. Press the 'Sample' button, and after setting a delay of seconds below the display screen, the measurement time starts. When the timer is complete, the display screen shows the sample absorbance increment (Δ Α) and inhibition rate, and prompts whether it is qualified or exceeded. The data can be automatically saved, and if necessary, press the 'Print' button to print.