In fields such as molecular biology experiments, clinical testing, and drug development that require high-precision liquid handling, the operation of pipettes directly affects the reliability of experimental data.As one of the mainstream laboratory tools, the Gilson electric pipette greatly reduces human error and improves pipetting efficiency due to its electric drive, precise quantification, and easy operation.Mastering its standardized usage method is a necessary skill for every experimenter.

　　1、 Preparation before use: matching and checking

Firstly, select an electric pipette with a suitable range according to the experimental requirements (such as the commonly used models by Gilson, such as 0.1-2 μ L, 2-20 μ L, 20-200 μ L, 100-1000 μ L, etc.), to ensure that the range covers the target liquid volume. Check if the appearance of the pipette is intact and if the battery is fully charged. When installing the suction head, align the pipette vertically with the matching suction head, gently press until you hear a "click" sound, and confirm that the suction head is securely installed without tilting. If different ranges are used, the target volume needs to be set through the range adjustment knob on the top of the pipette.

　　2、 Liquid suction operation: Vertical and uniform speed are key

1. Installing the suction head and pre rinsing: After installing the suction head, if it absorbs viscous liquids or easily residual reagents, it is recommended to pre rinse first - absorb the target liquid once and empty it, repeat 2-3 times, so that the inner wall of the suction head is fully wetted, to avoid liquid hanging on the wall and causing insufficient actual suction volume.

2. Immersion in liquid: Align the pipette vertically with the liquid container and keep the pipette tip 2-3mm below the liquid level. For liquids that are prone to foaming, the immersion depth can be increased to 5mm, but it needs to be kept vertical.

3. Press suction: Gently press the "suction button" on the top of the pipette with your thumb, and the pipette will automatically suction the liquid with the set suction force. At this time, keep the pipette vertically stationary for 1-2 seconds, ensuring that the liquid is completely filled with the pipette tip and there are no bubbles.

4. Transfer liquid: Keep the pipette vertical, slowly remove the suction head from the liquid surface, then press the suction head vertically against the inner wall of the target container, and gently press the "drain button" to release the liquid. If you need to accurately blow out residual liquid, you can long press the "drain button" to the second gear to ensure that all the liquid in the suction head is drained.

　　3、 Attention to detail: Accurate guarantee of details

1. Avoid cross contamination: The suction head must be replaced before each new liquid is drawn in; When using the same liquid continuously, it is recommended to replace the suction head every 10-15 times to prevent residue from affecting subsequent operations.

2. Environmental adaptation: When operating in fume hoods or biosafety cabinets, be careful to avoid air flow interference caused by rapid opening and closing of cabinet doors; Under high temperature conditions, the volume of liquid may expand, and it is necessary to reduce the preset volume appropriately.

3. Maintenance and Cleaning: Disassemble the pipette tip promptly after use and wipe the outer wall of the pipette with 70% ethanol; Regularly clean the nozzle cone with specialized cleaning agents; Remove the battery when not in use for a long time and store it in a dry environment.

4. Calibration and Inspection: It is recommended to calibrate the pipette every 3-6 months using standard weights or distilled water weighing method to ensure that the actual pipette volume is consistent with the set value.

From vertical immersion to precise drainage, the operation of the Gilson electric pipette combines "intelligent drive" and "fine control". Only by following standardized procedures and paying attention to detail management can this "liquid transfer assistant" truly become an efficient and reliable experimental partner in the laboratory.