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Zeiss polarizing microscope enhances your pathological diagnosis and research efficiency
Date: 2024-07-24Read: 0

Zeiss polarizing microscope enhances your pathological diagnosisAnd researchefficiency

The microscope isPathologistTools for daily diagnosis and research,Conventional microscopes can meet most of the usage needs,butInneeddiagnosisStarch likechangeandgout,evaluatefibrosisorganizationRepair degreeandIdentification of drug componentsisochronousPolarized microscopecanyesYour trusted assistant.

Let usExperience through several casespolarizing microscopeIn the clinical fieldofAmazing power!

1. Starch like transformation detection

Congo red staining is the gold standard for detecting tissue amyloidosis.As shown in the figure1-a,Using a Zeiss polarizing microscope, you can quickly locate the brick red amyloid protein in tissues under bright field observation.As shown in the figure1-b,Afterwards, using polarization, you can observe the bright and sharp apple green color of amyloid protein in an almost completely black tissue background, thus quickly and accurately determining its deposition on the tissue.

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Figure 1: Detection of amyloidosis in renal tissue slices.

2. Observation of collagen fibersAnd analysis

Sirius red staining is a commonly used method for observing and analyzing collagen fibers in tissues.As shown in Figure 2-a,Using a Zeiss polarizing microscope,The heart tissue slice samples stained with Sirius Red showed a completely black background tissue under orthogonal polarization,Collagen fibers can be clearly observed,among whichorangeandbright redfeatureFor type L collagen fibers, greenfeatureType III collagen fibers;As shown in Figure 2-b,Through Zeiss ZENThe software can quickly analyze the proportion of collagen fibers as 6.68%.

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Figure 2:Collagen fibers in cardiac tissue slicesObservation and Analysis.

3. crystalObservation and Analysis

The gold standard for diagnosing gout is the discovery of urate crystals through needle aspiration biopsy, and the diagnosis of diseases such as urinary tract infections and joint lesions also requires observation of crystals.As shown in the figure3-a,It is difficult to observe under a conventional microscopekidneyCrystals in the sample;As shown in the figure3-b,When observed with a polarizing microscope, the background of the visual field tissue is completely black, with oxalate crystalsvery obvious;As shown in the figure3-c,Add appropriate accessories to the polarized light pathIt can be clearly observed that oxalate crystals are distributed in the renal tubules.

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Figure 3:HEDetection of oxalate crystals in stained kidney tissue sections.

Zeiss polarizing microscope helps you easily observe weak birefringence features and present feature details. Rich polarization accessories enable us to provide professional polarization solutions tailored to your application scenarios.

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