Stable isotope technology is being used“Metabolic probe”The innovative role reshapes the cognitive boundaries of animal metabolism research. Its core advantage lies in breaking through the limitations of traditional static analysis, relying on the natural compatibility between labeled substrates and endogenous molecules in organisms to achieve real-time dynamic tracking of metabolic processes, multidimensional flux quantification, and unknown reaction mining. It provides a highly accurate and interference free research tool for analyzing normal physiological metabolism and disease-related metabolic disorders in animals, and has become a key technical bridge connecting overall phenotype and molecular mechanisms.

Literature 1: Calculate the whole body'Daily account'——Cell Metabolism 2025

Harvard University teampassbuild“Mouse Whole Body Energy Flux Model”，quantification10Comparison of oxidation, storage, recycling, and interconversion rates of major circulating nutrients了lean（Normal diet group）TheHFD（High-fat diet group）andleptindefectob/obmouse（A model of congenital leptin deficiency, extreme obesity, and severe hyperglycemia）Metabolic economic differences.

experimentunite¹³C-Substrate infusion, serumLC-MSandStable isotope analyzerYes, that's right10Main Nutrients“revenue and expenditure”Inventory: Circulating lactate96%Oxidized, while glucose is only81%Essential amino acids only30%Left and right, showing the inherent presence of different substrates“oxidation/store”Allocation ratio; InleanAll nutrients in mice“Invalid loop”Flux (glycogen)-Glucose% s's mixThe total protein turnover is higher than the oxidation flux23%Waste about9%The overallATP；ob/obThe overall circulation flux of mice increased2Double,ATPIncreased waste12%ButHFDMice andleanThere is not much difference, promptCausing metabolismThe key reason for the overactive "ineffective circulation" and excessive energy waste is the deficiency of leptin, a hormone, rather than simply weight gain or excess fat. In the clinical treatment of certain metabolic syndromes, correcting the "leptin signal deficiency" is more crucial than simply "weight loss".

Reference 2: Glucose ConversionChase "to organelles——Nature Communications 2025

VanderbiltteamIn《Nature CommunicationsHow to translatePublished an article研究achievements,Continuous intravenous infusion to conscious micehighvslow dose[U-¹³C ₆]-Glucose, they found:High dose group（40mg）¹³60mininsideCO2Exhaling reached the plateau period, while blood was drawn to measure glucose isotope labeling, and it was found that680%¹³Glucose moleculesAll carbon is consumedCReplace（30M+6）Take out the liver and measure glycogen, and find that there are glucose units in it–¹³50%yes¹³CMarked, proving that it is "ready-made" from the bloodC-Glucose is directly captured by the liver to synthesize glycogen, with a very fast synthesis rate; combineMALDI-MSImagingMIMS-EMWaiting for technology,This study will“organ-cell-organelle

”The three-level metabolic map forms a timeline.Literature 3: Is exercise time important? 2024

---Metabolismin order toExploring the effect of exercise time (circadian rhythm) on substrate metabolism after exercise, and how obesity changes this“time-metabolism”plasticity，KarolinskaWith Ge BenhaThe Root TeamleanandHFDMice in the resting phase（ZT3）Or activity period（ZT15）Running platform60min₆Subsequently, gavage was administered[U-¹³C]-2Glucose and continuous measurement

¹³CO呼出。The results show thatLeanMice only exhibit activity after exercise during the active phase¹³C-The peak of glucose oxidation decreases and lipolysis increases, showing“time-exercise”Dual effect;HFDMice lose their diurnal differences, and exercise time no longer affects the oxidation curve, indicating that obesity leads to impaired metabolic plasticity;sutraAdipose tissue in vitro experimentfurther

Indicating that the improvement effect of exercise on metabolism depends on the day and night time periods.

Beijing Yiketai Ecological Technology Co., LtdAs a professional technical service provider specializing in animal metabolism research, with over ten years of industry accumulation and international technical cooperation resources, it has become the core promotion and technical support force for animal stable isotope related equipment in China. The stable isotope gas analysis system provided by the company has:lHigh precision detection:0.6δ¹³C

Precision achieved‰lMulti parameter synchronous monitoring: synchronous measurement¹³CO ₂TheH2OWaiting for key indicators,Combined with oxygen consumptionVO2、Carbon dioxide productionVCO2Unlock metabolic processes with multi-dimensional parameters

ofoverall picturelFast response：

1Hzmeasure frequency

lNo additional consumables required for calibrationthe systemCan be flexibly matchedRespiratory metabolic system、

Small animal treadmill

Waiting for equipment to form a complete solution from overall metabolism to substance oxidation tracking, efficiently conducting innovative research in animal physiology, ecology, biomedical and other fields.

References:

[1] Habashy A, Acree C, Kim K-Y, et al. Spatial patterns of hepatocyte glucose flux revealed by stable isotope tracing and multiscale microscopy [J]. Nature Communications, 2025, 16: 5850. DOI:10.1038/s41467-025-60994-w.

[2] Yuan B, Doxsey W, Tok Ö, et al. An organism-level quantitative flux model of energy metabolism in mice [J]. Cell Metabolism, 2025, 37(4): 1-12. DOI:10.1016/j.cmet.2025.01.008.