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Laminar flow washing system: multidimensional functional analysis
Date: 2025-11-17Read: 0
As a core equipment in the field of life sciences, laminar flow washing system has achieved cross scenario applications from cell processing to environmental cleaning through innovative fluid control technology. Its core functions cover four modules: staining, incubation, cleaning, and automated operations. Combined with specially designed consumables and intelligent software, it provides efficient and accurate solutions for fields such as flow cytometry, single-cell sequencing, and industrial cleaning.
1、 Dyeing function: an automated engine for high-throughput experiments
The laminar flow washing system supports the full process automation of cell surface and intracellular staining through pre installed modifiable experimental method libraries. Taking the Curiox AUTO1000 system as an example, its built-in 96 well plate is designed with hydrophilic points and hydrophobic walls, allowing suspended cells to accurately stay at each hydrophilic point and avoid cross contamination. Users can quickly set parameters such as antibody volume and washing times through an intuitive software interface. The system performs well in immunophenotyping analysis.
2、 Incubation Function: Gentle Guardian of Cell Activity
The traditional centrifugation method often leads to a decrease in cell activity due to mechanical stress, while the laminar flow washing system provides a mild incubation environment for cells through gravity settling and laminar flow buffering technology. The Curiox HT2000 system achieves a wide temperature range of 4-70 ℃ incubation in a 96 well plate, coupled with a hydrophobic enclosure design, allowing cells to maintain their intact form without centrifugation during the washing process. Experimental data shows that the activity of TILs (tumor infiltrating lymphocytes) processed by the system increased by more than 30%, and the fragmentation rate decreased by 50%, making it particularly suitable for the preparation of rare cell samples.
3、 Cleaning function: the core breakthrough of laminar flow technology
The laminar flow washing system achieves efficient and non-destructive cell cleaning through dual nozzle symmetrical suction technology. Taking the MINI 1000 system as an example, its nozzle distributes washing buffer in parallel laminar flow, while simultaneously absorbing waste liquid and debris. It only takes 2 minutes to complete dye removal in 7 cycles. In single-cell sequencing applications, this technology has increased the cell recovery rate from 19% by centrifugation to over 50%, and the unbound antibody clearance rate has reached 99%, significantly optimizing data quality.