Semi-automatic enzyme-linked immunosorbent assay analyzerIt is a detection device based on the principle of enzyme-linked immunosorbent assay (ELISA), mainly used for qualitative and quantitative analysis of analytes in biological samples. The basic working principle is to form an antigen antibody complex by specifically binding enzyme labeled antibodies to antigens in the test sample. Subsequently, specific substrates are added to react with enzymes, producing color changes or fluorescent signals. These signals are directly proportional to the concentration of the analyte, so they can be quantitatively analyzed through photoelectric colorimetry or fluorescence detection methods to achieve qualitative and quantitative detection of specific components in the sample.
Semi-automatic enzyme-linked immunosorbent assay analyzerMain functions:
1. Absorption detection (core function)
Basic principle: This is the most fundamental and commonly used function of an enzyme-linked immunosorbent assay (ELISA) reader. It illuminates the sample in the microplate with a specific wavelength light source to detect the degree of light absorption (i.e. absorbance value, OD value) of the sample.
Application: Mainly used for colorimetric detection, it is the standard detection method for ELISA testing. By measuring the color intensity (absorbance) of reaction products, the concentration of target substances such as antigens, antibodies, proteins, nucleic acids, cytokines, hormones, and drug residues can be quantitatively analyzed.
Wavelength selection: Typically equipped with multiple fixed filters (such as 405nm, 450nm, 492nm, 630nm, etc.), users can manually select the appropriate detection wavelength according to their experimental needs.
2. Microplate reading
Core task: Able to quickly and batch read optical signals from standard 96 well plates (the most common), 48 well plates, 24 well plates, etc.
Reading mode: Supports multiple reading modes such as single wavelength and dual wavelength (used to deduct background interference).
3. Data Processing and Analysis
Built in computing: Instruments are usually equipped with display screens and simple operating systems, which can perform basic data processing, such as:
Calculate the mean and standard deviation.
Perform standard curve calculations such as linear, quadratic, and four parameter fitting.
Automatically calculate the concentration of unknown samples based on the standard curve.
Result output: Raw data and analysis results can be transmitted to a computer through interfaces such as USB and RS232, or directly connected to a printer to output reports.
4. Oscillatory mixing function
Built in oscillator: Many semi-automatic enzyme-linked immunosorbent assay (ELISA) analyzers have built-in oscillation functions (linear oscillation, circular oscillation, or orbital oscillation) at the bottom or platform.
Function: Before reading, the microporous plate can be briefly oscillated to thoroughly mix the liquid in the pores, ensure uniform reaction, and improve the accuracy and repeatability of the detection results.
5. Incubation function (some models)
In plate incubation: Some advanced semi-automatic enzyme-linked immunosorbent assay (ELISA) analyzers have incubation functions, which can heat the microplate and maintain it at a specific temperature (such as 37 ° C).
Application: Used for immune or enzymatic reactions that require constant temperature conditions, reducing reliance on additional incubators and improving experimental efficiency.
The manifestation of 'semi-automatic'
Compared with the fully automatic enzyme-linked immunosorbent assay (ELISA) reader, the "semi-automatic" of the semi-automatic ELISA reader is mainly reflected in:
Manual sample addition: The addition of samples and reagents relies on a manual pipette.
Manual placement: Users need to manually place the microplate into the tray of the instrument.
Manual selection: wavelength selection, program settings, etc. usually require users to manually operate on the instrument panel.
No automated processing: does not have highly automated functions such as automatic plate washing, automatic sampling, and robotic arm grasping.