PT full wavelength enzyme-linked immunosorbent assay (ELISA) readerIt is a high-precision analytical instrument that integrates multiple detection modes such as UV visible absorption, fluorescence, chemiluminescence, etc. It is widely used in fields such as life sciences, clinical diagnosis, drug development, and food safety. Its core advantages lie in wide wavelength coverage (usually 190-1000nm) and multifunctional detection capabilities, which can meet diverse needs from basic scientific research to industrial production. It is based on the law of light absorption (Bill Lambert law), emitting light of a specific wavelength through a light source. After being absorbed by the sample, the detector measures the remaining light intensity and calculates the absorbance (A) to determine the concentration of the target molecule.

　　PT full wavelength enzyme-linked immunosorbent assay (ELISA) readerThe core technical features are as follows:

1、 Full wavelength coverage, wide detection range

Wide wavelength range: usually covering 200nm-1000nm (UV Vis NIR region), it can meet the characteristic absorption detection needs of different substances. For example:

Nucleic acids (DNA/RNA) have characteristic absorption at 260nm;

Protein detection at 280nm;

Readings at wavelengths such as 450nm and 630nm in colorimetric reactions (such as ELISA);

Flexible selection of excitation/emission wavelengths in fluorescence detection (such as fluorescence probe labeling).

Continuous spectral scanning: supports full wavelength scanning of a single sample well, generating absorption spectrum curves for easy analysis of substance purity (such as nucleic acid purity can be determined by the 260/280nm ratio) or screening of the optimal detection wavelength.

2、 High precision optical system with high detection sensitivity

Grating monochromator technology: Using high-quality gratings as spectral elements, it has higher wavelength accuracy (within ± 1nm) and bandwidth adjustability (usually 0.5-10nm adjustable) compared to filter based enzyme-linked immunosorbent assay (ELISA), which can reduce stray light interference and improve detection specificity.

High sensitivity detector: equipped with photomultiplier tube (PMT) or CCD array detector, capable of capturing weak light signals (such as fluorescence or chemiluminescence of low concentration samples), with a detection limit of up to pg level (some models), meeting the needs of trace sample analysis.

Optical path calibration function: Built in automatic calibration module, regularly calibrates the optical path, wavelength accuracy, and stability to ensure the repeatability of long-term detection data (CV value is usually less than 1%).

3、 Diversified detection modes, comprehensive functions

Basic detection mode:

Absorption light detection (colorimetric method): suitable for ELISA, enzyme activity assay, cell proliferation (such as MTT method), etc;

Fluorescence detection: supports top/bottom excitation, can detect fluorescence intensity (FI), fluorescence polarization (FP), time-resolved fluorescence (TRF), etc., used for fluorescence probe labeling, homogeneous time-resolved fluorescence immunoassay (HTRF), etc;

Chemiluminescence/Bioluminescence Detection: Without the need for light source excitation, it can directly detect the light signals released by chemical reactions or biological systems (such as luciferase reporter genes) with high sensitivity.

Advanced application mode:

Dynamics detection: Real time monitoring of signal changes in the reaction system over time (such as enzyme kinetics curve, cell growth curve), supporting temperature control (25-45 ℃) and oscillation function (ensuring reaction uniformity);

Pore scanning: perform multi-point scanning on a single well of a 96 well plate (such as detecting whether the sample inside the well is uniform), or perform targeted analysis on specific areas (such as cell clones);

Flexible switching between endpoint method/kinetics method: meets different experimental design requirements (such as endpoint method for qualitative/quantitative analysis, kinetics method for reaction rate analysis).

4、 Automation and high-throughput compatibility, high efficiency

Compatible with multiple microplates: Supports 6-1536 well plate detection, with 96 well plate being the standard specification and 384/1536 well plate suitable for high-throughput screening (such as drug candidate compound library screening), improving experimental efficiency.

Automatic sampling and rapid reading: Equipped with a robotic arm for automatic plate feeding (some models), the reading time for a single 96 well plate can be as short as 10 seconds, and tens of thousands of tests can be completed daily in high-throughput mode.

Integrated temperature control and oscillation: Built in high-precision temperature control module (temperature difference ± 0.1 ℃), can simulate physiological environment (such as 37 ℃ cell culture conditions); Support linear and orbital oscillation modes to ensure uniform sample mixing and reduce detection errors.

5、 Intelligent operation and data management

Friendly software system: equipped with a graphical user interface, supports preset detection schemes (such as ELISA, nucleic acid quantification and other standard programs), and can also customize detection parameters (wavelength, time, temperature, etc.), making it easy for beginners to get started.

Powerful data processing capabilities: automatically calculate concentrations, ratios (such as 260/280), kinetic parameters (such as Km, Vmax), and generate curves and reports; Support data export (in Excel, PDF, and other formats) and integration with LIMS system.

Multi channel parallel detection: Some high-end models use multi-channel detection technology (such as 8-channel) to simultaneously read multiple hole positions, reducing detection time while ensuring consistency between channels (CV<0.5% between channels).

6、 Stability and durability design

Strong anti-interference ability: The optical system adopts a sealed design to reduce the impact of ambient light and temperature fluctuations on detection; Some models are equipped with shock-absorbing bases to reduce external vibration interference.

Easy maintenance: The core components (such as gratings and detectors) have a long lifespan, and daily maintenance only requires cleaning the sample chamber and calibrating the optical path, without frequent replacement of consumables, reducing usage costs.