Microplate ReaderIt is a laboratory equipment used for detecting and analyzing optical signals of samples, widely used in fields such as life science research, clinical testing, drug development, and food safety testing. Its working principle is mainly to quantitatively analyze the content of target molecules or biological components in the sample by detecting the absorption, emission, or reflection signals of light in the sample. Enzyme linked immunosorbent assay (ELISA) is particularly suitable for high-throughput experiments, usually used in conjunction with 96 well, 384 well, or even 1536 well microplates, and can detect a large number of samples at once.

　　Microplate ReaderOperation steps:

1. Preheating and Calibration: Preheat the machine for 20-30 minutes and calibrate the wavelength and absorbance range using standard solution.

2. Sample processing: Dilute the sample according to experimental requirements and control the pH value.

3. Sample addition and incubation: Add samples, enzyme-linked antibodies, and substrates to the microplate, and incubate at a constant temperature (such as 37 ℃, 1 hour) to promote the reaction.

4. Termination reaction and reading: After adding the termination solution, immediately use an enzyme-linked immunosorbent assay (ELISA) reader to detect the absorbance and avoid color fading.

5. Data analysis: Calculate sample concentration using standard curve method or perform statistical analysis using software.

Enzyme linked immunosorbent assay (ELISA) is widely used as an analytical instrument in biomedical research, food safety monitoring, and environmental analysis, mainly for measuring and analyzing absorbance in experiments such as enzyme-linked immunosorbent assay (ELISA). Its working principle is based on the absorption of light by the products generated by chemical reactions at specific wavelengths, and then the concentration of the substance to be tested in the sample is calculated by measuring the absorbance.