Shimadzu GC2010 gas chromatographThe pulse non splitting injection technology is an efficient sample injection method, and the following are the main technical points of this technology:

1、 Overview of Injection Methods

Pulse non split injection is the process of closing the split flow path at the moment of injection, allowing the sample to enter the chromatographic column at a higher carrier gas flow rate, thereby increasing the amount of sample entering the column and improving detection sensitivity. After the injection is completed, open the diversion flow path again to restore the normal diversion injection state.

2、 Detailed explanation of technical points

1. Split flow control at the moment of injection:

During injection, by precisely controlling the switch of the diversion flow path, it is ensured that the sample enters the chromatographic column instantly without diversion.

This requires high-precision solenoid valves and flow control systems to achieve.

2. Adjustment of carrier gas flow rate:

During the pulse non split injection process, the carrier gas flow rate needs to be precisely adjusted to ensure that the sample can be fully vaporized and enter the chromatographic column.

A too fast carrier gas flow rate may cause the sample to fail to vaporize, while a too slow flow rate may cause the sample to diffuse in the chromatographic column.

3. Relationship between injection volume and split ratio:

When pulse injection is not split, the injection volume is usually large, so it is necessary to set the split ratio reasonably to avoid column overload.

The setting of the diversion ratio needs to be determined based on the properties of the sample and the detection requirements.

4. Protection of chromatographic columns:

During the pulse non split injection process, due to the large sample size, it may cause some impact on the chromatographic column.

Therefore, appropriate protective measures need to be taken, such as using pre columns, protective columns, etc., to extend the service life of the chromatographic column.

5. Maintenance of injection port:

The injection port isShimadzu GC2010 gas chromatographOne of the parts that are easily contaminated.

It is necessary to regularly replace consumables such as injection pads, liners, O-rings, and clean components such as the vaporization chamber to ensure the normal operation of the injection port and improve the stability of the instrument.

3、 Application precautions

1. Sample pretreatment:

Before conducting pulse non split injection, it is necessary to perform appropriate pre-treatment on the sample, such as dissolution, dilution, filtration, etc., to ensure that the sample can smoothly enter the chromatographic column and obtain accurate detection results.

2. Calibration and validation of instruments:

When using pulse non split injection technology, it is necessary to regularly calibrate and validate the instrument to ensure the accuracy and reliability of the detection results.

3. Training for operators:

Operators need to receive professional training to understand the principles, operating methods, and precautions of pulse non splitting injection technology, in order to ensure the correct use and maintenance of the instrument.

The pulse non split injection technology of Shimadzu GC2010 gas chromatograph requires precise control of the switch of the split flow path, adjustment of the carrier gas flow rate, reasonable setting of the split ratio, and appropriate column protection measures. At the same time, attention should be paid to application precautions such as sample pretreatment, instrument calibration and validation, and operator training.