Dr. Jiang Shengwei from Sun Yat sen Medical College, Sun Yat sen University, tested the sample that should have been discarded with a terminal reagent after completing the organoid drug screening experiment, and unexpectedly found that it could secrete insulin. This phenomenon has triggered a series of questions: Will a micro tissue cultured in vitro affect systemic metabolism? The team subsequently validated in db/db mice and found that after a single subcutaneous implantation, blood glucose levels steadily decreased for up to a month.

Oh, isn't that right? "Many people have had similar thoughts. But Dr. Jiang and his team chose to ask: If it can secrete insulin, will it affect systemic metabolism? So, they designed a complete set of validation experiments by subcutaneously implanting organoids into db/db mice, and then using multiple omics methods such as metabolomics, proteomics, and single-cell sequencing to systematically observe the response of the liver, fat, and muscle tissues.

In this issue of 'Science Speaks', we invite Dr. Jiang Shengwei to reminisce and document the complete research path from' What? 'to' Aha! 'together. There is no predetermined answer, only respect for abnormal data, adherence to verification logic, and honest sharing of how technical tools support discovery.