The Agilent Altura Poroshell HILIC-Z chromatographic column, utilizing ultra-high inertia technology, has been carefully designed and manufactured to achieve reverse phase separation of small molecule polar analytes such as amino acids and organic acid metabolites. The zwitterionic stationary phase bonded on the surface porous silica gel particles can quickly, efficiently, and reproductively separate charged small molecules. The coated stainless steel chromatography column hardware ensures surface inertness, significantly improves the recovery rate of anionic analytes, and has the durability and consistency of stainless steel.