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Product Categories
- CO2 constant temperature incubator
- CO2 incubator
- CO2 shaker
- reagent
- IPS preparation of reprogramming reagents
- Mesenchymal stem cell culture medium
- Chemical composition limited culture medium
- Platelet lysate
- mesenchymal stem cell
- Digital scanning microscopy imaging system
- IPS differentiation reagent
- Organoid culture kit
Nanjing Lanbo Aix Biotechnology Co., Ltd
mesenchymal stem cell
NegotiableUpdate on 01/30
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Overview
Mesenchymal stem cells (adipose tissue, bone marrow, endometrium, umbilical cord, gingiva, etc.)
Product Details
| source | Fat, bone marrow, endometrium, umbilical cord, gums, etc |
|---|---|
| form | Fibroblast like cells with elongated spindle shaped and spiral shaped adherent growth |
| cultivate | Incubator at 37 ℃ and 5% CO2 |
| subculture | (1) When the cell density reaches 80% -90%, passage can be carried out (note that the cells cannot be too dense, otherwise they are prone to differentiation). Discard the culture supernatant and rinse the cells 1-2 times with DPBS that does not contain calcium or magnesium ions; (2) Add 1mL of recombinant pancreatic tissue to a culture flask, allowing the digestive fluid to infiltrate all cells. Place the cells in a 37 ℃ incubator for digestion for 1-3 minutes and observe under a microscope until most of the cells become round and shed (depending on the cell condition). (3) After addition, transfer to a sterile centrifuge tube and centrifuge at 1000 rpm for 5 minutes. Discard 3 ml of MSC medium to terminate digestion. Gently mix and remove the supernatant, then add 1-2 ml of MSC medium and blow evenly. (4) Passage should be carried out in a ratio of 1:3 or 1:4, or cells should be counted and seeded into new culture bottles at a concentration of 5-8 × 103 cells/cm2. After supplementing with the corresponding volume of MSC culture medium, the cells should be cultured in a 37 ℃, 5% CO2 incubator. (5) Change the medium every 2-3 days and culture for 2-4 days until the cell growth density reaches around 80% before proceeding with subsequent passaging or cryopreservation operations. |
| Product Introduction | umbilical cordmesenchymal stem cell(hUC MSCs) are a type of stem cell with multipotent differentiation ability extracted from the umbilical cord of newborns. The source of umbilical cordmesenchymal stem cellNot only does it maintain the biological characteristics of mesenchymal stem cells (surface antigen markers, with the ability to differentiate into osteoblasts, chondrocytes, and adipocytes), but it also outperforms other sourcesmesenchymal stem cellSafer, more primitive, with stronger cell proliferation, differentiation, and immune regulation abilities. In addition, the umbilical cordmesenchymal stem cellIt also has the characteristics of convenient sampling and easy separation, low immunogenicity, no ethical and moral restrictions, a large number of available cells, and the ability to achieve large-scale standardized production, with broad clinical application prospects. This product has undergone strict quality control and testing to ensure its safety and effectiveness. |
| Receiving Guide | (1) Inspection: label on the culture bottle, integrity of the culture bottle, and whether there is any leakage at the bottle mouth; (2) Disposal: After disinfecting the culture bottle with 75% alcohol, place it in a 37 ℃, 5% CO2 incubator for 2-4 hours to stabilize the cell state, and conduct microscopic observation and photography as a basis for after-sales rights protection; |
| Cultivation methods | |
| Biosafety level | 1 |
| Disclaimers | This product is only for scientific research purposes and should not be used for clinical diagnosis, treatment, or other purposes. Users should comply with national and regional laws and regulations, and bear all risks and responsibilities arising from the use of this product on their own. Please read this manual carefully before use and follow the instructions for operation. If you have any questions or need further information, please contact us. |
