Zebrafish alanine aminotransferase (ALT) ELISA detection kit enzyme-linked immunosorbent assay

1、 Product Name

Zebrafish alanine aminotransferase (ALT) ELISA detection kit enzyme-linked immunosorbent assay
Item No.MO-Q91410T

2、 Packaging specifications

96 wells/box (including complete set of components such as standards, reagents, etc., capable of detecting 90 samples+6 standards)

3、 Expected use

This kit is used for quantitative detection of ALT concentration in zebrafish serum, plasma, tissue homogenate, cell supernatant and other samples in vitro, and can be used in fields such as immunology research.

4、 Detection principle

Based on double antibody sandwich enzyme-linked immunosorbent assay (ELISA).

1. Coated Plate: The microporous plate is pre coated with anti human ALT monoclonal antibody (capture antibody), which can specifically bind to ALT in the sample.
2. Sample incubation: Add the test sample, and the ALT in the sample binds to the coated antibody.
3. Antibody detection: Add biotinylated anti ALT polyclonal antibodies (detection antibodies), bind to another epitope of ALT, and form a "coated antibody ALT detection antibody" complex.
4. Enzyme labeled avidin: Add streptavidin horseradish peroxidase (HRP) complex, bind with biotin, and amplify the signal.
5. Color reaction: Add substrate solution (TMB), HRP catalyzes substrate color development, and the color depth is proportional to ALT concentration.
6. Termination and Reading: Add termination solution to terminate the reaction, measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the concentration of ALT in the sample using a standard curve.
   

   Precautions

   1. The reagent kit needs to be equilibrated to room temperature (20-25 ℃) before use to avoid condensation affecting the results.
   2. Avoid bubbles when adding samples, and calibrate the pipette to ensure accurate sample addition.
   3. Insufficient washing can result in a high background, and the washing solution should cover the micropores. When discarding the solution, pat dry (to avoid residue in the pores).
   4. TMB substrates need to be stored away from light, and the color development time needs to be strictly controlled (too long may result in a darker color).
   5. If the sample is frozen, it is necessary to avoid repeated freezing and thawing (it is recommended to pack and store).
   6. In the experiment, blank controls (only standard dilution solution) and negative controls (known low concentration samples) need to be set up to verify the effectiveness of the experiment.
   
   

   Applicable sample types

   Serum, plasma (EDTA/heparin anticoagulant), tissue homogenate, cell culture supernatant, cerebrospinal fluid and other body fluid samples.
   Zebrafish alanine aminotransferase (ALT) ELISA detection kit enzyme-linked immunosorbent assay
   This kit is only used for scientific research and is not intended for clinical diagnosis or treatment.
   
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