Ultra-high resolution optical microscope

Ultra-high resolution optical microscopeDesign concept:

1. Provide a wide range of imaging modalities to enhance the applicability and functionality of Multi SIM samples, allowing one device to meet more experimental needs TIRF-SIM、GI-SIM、Single Slice-SIM、Stacked Slices-SIM、3D-SIM、Nonlinear-SIM、TIRF、GI、Oblique、WF、BF、DIC、Phase Contrast、Single molecule tracking Wait.

2. Larger imaging field of view, faster imaging speed, four channel simultaneous imaging and high-speed switching, multi-point shooting, puzzle shooting, etc., high-speed data acquisition and storage, improve experimental efficiency, and collect more samples with super-resolution details.

3. Based on artificial intelligence fluorescence image processing algorithms, both image denoising and computational super-resolution are far superior to traditional algorithms.

TIRF-SIM cell membrane.

GI-SIM cytoplasmic organelles.

Single Slice SIM is a single layer within cells.

Stacked Slices SIM cells are stacked at multiple levels within the cell.

3D-SIM whole cell.

The Stacked Slices SIM modality involves stacking multiple layers within cells, allowing for the acquisition of three-dimensional information of the sample at a lower cost.

3D-SIM mode is a three-dimensional super-resolution mode that uses three-dimensional structured light to illuminate the sample, thus providing twice the super-resolution capability in the axial direction. The axial resolution is increased to 350nm, allowing for the acquisition of all three-dimensional super-resolution information of the sample.

The single molecule tracking mode can perform single molecule dynamic tracking and can be associated with super-resolution imaging.

Nonlinear SIM mode, also known as non-linear SIM mode, uses a special imaging scheme to increase the resolution of structured light illumination imaging to three times that of wide field imaging, reaching 60nm, which is also our core technology.

Single molecule tracking， That is to say, single-molecule tracking mode is a new mode that we expect to release in early 2023. It can be effectively associated with super-resolution imaging, tracking the motion process and characteristics of molecules while performing super-resolution imaging.

Ultra-high resolution optical microscopeIn addition to the multimodal design concept, another feature of Multi SIM is high-throughput super-resolution microscopy. Therefore, the latest Multi SIM Zeiss version offers an ultra fast shooting mode.

Compared to the previous fast mode, the imaging speed has been further increased by 20%, from 564 fps to 687 fps. At the same time, the contradiction between imaging speed and imaging field of view has been resolved. Under ultra fast shooting, the imaging field of view has been increased from 72 * 512 pixels to 512 × 1536 pixels, expanding the imaging field of view by 21.3 times and achieving a gain of 25.6 times the information content before. Enabling experimenters to obtain more information with fewer shots and reducing the number of repetitive tasks required to capture images is truly high-throughput photography.