Transgenic soybean MON87705 strain nucleic acid test kit

Product NameGenetically modified soybeanMON87705 strain nucleic acid test kit (fluorescence PCR method)

25T/Box50T/box

【 Principle of Inspection 】This reagent kit usesTaqManReal time fluorescence using probe methodPCRTechnology, according toGenetically modified soybeanMON87705 seriesGene design specific primers and fluorescent probes, using fluorescencePCRTechnology forGenetically modified soybeanMON87705 seriesThe nucleic acid is subjected to in vitro amplification testing for pathogen diagnosis of suspected infectious materials in clinical practice.

Our company has a complete range of real-time fluorescent probe assay kits (covering poultry diseases, pig diseases, horse, cattle, sheep diseases, and aquatic animal pathogen detection), which are precise, efficient, and capable of multiple detection (single, double, triple),nowGoods supply welcomes new and old customers to comeconsultation andchoose.

【 Reagent Composition 】

Explanation: Components of different batch numbers of reagent kits cannot be used interchangeably

Storage conditions and expiration date

-20℃±5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles not exceeding5 Second, validity period 12 A month.

ABI7500Agilent MX3000P/3005PTheLightCyclerTheBio-RadTheEppendorfWaiting series fluorescence quantificationPCRDetector.

【Transgenic soybean MON87705 strain nucleic acid test kitInstructions for use

1. Sample processing (sample processing area)

nucleic acid extraction It is recommended to use nucleic acid extraction or purification reagents (magnetic bead method or centrifugal column method) for nucleic acid extraction. Please follow the instructions of the reagents for operation.

2. Reagent preparation (reagent preparation area)Based on the total number of samples to be tested, set the requiredPCRThe number of reaction tubes is N(N=sample size+1 Tube negative control+1 Positive control of the tube; When the number of reaction tubes is full10 Prepare multiple portions1 copy)The configuration of each test reaction system is shown in the following table:

Divide the mixed test reaction solution into separate packages PCRIn the reaction tube,20μL/Manage.

3. Sample addition (sample processing area)Step by step 1 Extract nucleic acid, positive control samples, and negative control samples separately5μLAdd them separately into the corresponding reaction tubes, cover the tube caps, mix well, and centrifuge briefly.

4. PCR amplification (nucleic acid amplification zone)

2.1 Place the reaction tube to be tested in fluorescence quantificationPCRInside the instrument reaction tank;

2.2 Set the channel and sample information, and set the reaction system to25μLFluorescence channel selection: detection channel（Reporter Dye）FAM, quenching channel（Quencher Dye）NONE，ABIPlease do not select the series of instruments ROXReference fluorescence, selection NoneJust enough.

2.3 Recommended loop parameter settings:

5. Result analysis and judgment

5.1 Result Analysis Condition Setting(Please refer to the instructions for each instrument to set up for analysis) ABI7500Taking instruments as an example)After the reaction is completed, the results will be automatically saved and adjusted based on the analyzed imageBaselineof StartValueEndValue and ThresholdValue (user can root)Adjust according to the actual situation,StartThe value can be set to 3～15TheEndThe value can be set to 5～20Place the threshold line at the exponential phase of the amplification curve and perform negative quality controlIf the amplification curve of the product is flat or below the threshold line, clickAnalyzeAutomatically obtain analysis results.

5.2 Result judgment

Positive: Detection channelCtvalue≤40And the curve has a clear exponential growth curve;

Negative: No sample test resultCtValue, and no specific amplification curve;

Suspicious: Sample testing results40＜Ctvalue≤45It is recommended to repeat the detection. If the detection channel is still40value≤45And the curve shows a significant increaseThe curve is judged as positive, otherwise it is judged as negative.

5.3 Quality Control StandardsNegative quality control product: No specific amplification curve or noneCtValue display; Positive quality control product: The amplification curve shows a significant exponential growth period, and Ctvalue≤32The above conditions should be met simultaneously, otherwise the experiment will be considered invalid.

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