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Shanghai Yingdong Biotechnology Co., Ltd
TRIzol reagent
NegotiableUpdate on 05/06
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Overview
TRIzol reagent is a complete, ready to use reagent that can isolate high-quality total RNA or simultaneously isolate RNA, DNA, and protein from multiple biological samples.
Product Details
specification
Separation technology
Organic extraction
Purification Time
An hour
Sample Type
Bacteria, blood, cells, plant samples, tissues, virus samples, yeast
Final product type
Total RNA, Transcriptome RNA, Micro RNA
High throughput capability
Not compatible with high-throughput applications (manual)
Applicable to (application)
Real time fluorescence quantitative PCR (qPCR), reverse transcriptase PCR (RT-PCR), cDNA library construction, nuclease protection assay, Northern blotting, cloning
including
200 mL TRIzol reagent
Number of reactions
200 preparations
product line
TRIzol ™
quantity
1
specification
Readable scale
transport conditions
room temperature
Raw material quantity
Up to 1 g of tissue, up to 1 x 10 ^ 7 cells
target
Total RNA, transcriptome RNA, miRNA
System Type
TRIzol
Content and Storage
One bottle. Store at room temperature.
TRIzol reagentIt is a complete and ready to use reagent that can isolate high-quality total RNA or simultaneously isolate RNA, DNA, and protein from multiple biological samples. The single-phase solution of guanidine isothiocyanate is designed to isolate individual components of RNA, DNA, and protein from cell and tissue samples of human, animal, plant, yeast, or bacterial origin within one hour.
TRIzol ™ The main characteristics of the reagent include:
Can separate RNA, DNA, and proteins from the same sample
• Possess greater cracking ability than Z, even capable of handling difficult sample types
• Formulas and protocols optimized for organizations, cells, serum, viruses, and bacteria
Reliable purification of RNA from various sample volumes and sources
TRIzol reagentCan effectively handle small amounts of tissue (50-100 mg) and cells (5 × 10)6）And a large number of tissues (≥ 1 g) and cells (>10 g)7）And accompanied by a purification scheme from samples derived from humans, animals, plants, or bacteria. TRIzol ™ Reagents maintain RNA integrity by efficiently inhibiting RNase activity during sample homogenization, while disrupting cells and dissolving cellular components. TRIzol ™ The simplicity of the reagent method allows it to process a large number of samples simultaneously. The entire program can be completed within 1 hour. Through TRIzol ™ The total RNA isolated by the reagent does not contain protein or DNA contamination.
Its formula can separate multiple molecular targets
TRIzol reagentIt allows you to continuously precipitate RNA, DNA, and proteins from a single sample. Using TRIzol ™ After homogenizing the sample with reagents, chloroform was added to separate the homogenate into a transparent upper water layer (containing RNA), an intermediate phase, and a red lower organic layer (containing DNA and protein). Use isopropanol to precipitate RNA from the aqueous layer. Use ethanol to precipitate DNA from the intermediate phase/organic layer. Proteins were precipitated from the ethanol supernatant by isopropanol precipitation. Wash the precipitated RNA, DNA, or protein to remove impurities, and then resuspend for downstream applications.
TRIzol ™ The main characteristics of the reagent include:
Can separate RNA, DNA, and proteins from the same sample
• Possess greater cracking ability than Z, even capable of handling difficult sample types
• Formulas and protocols optimized for organizations, cells, serum, viruses, and bacteria
Reliable purification of RNA from various sample volumes and sources
TRIzol reagentCan effectively handle small amounts of tissue (50-100 mg) and cells (5 × 10)6）And a large number of tissues (≥ 1 g) and cells (>10 g)7）And accompanied by a purification scheme from samples derived from humans, animals, plants, or bacteria. TRIzol ™ Reagents maintain RNA integrity by efficiently inhibiting RNase activity during sample homogenization, while disrupting cells and dissolving cellular components. TRIzol ™ The simplicity of the reagent method allows it to process a large number of samples simultaneously. The entire program can be completed within 1 hour. Through TRIzol ™ The total RNA isolated by the reagent does not contain protein or DNA contamination.
Its formula can separate multiple molecular targets
TRIzol reagentIt allows you to continuously precipitate RNA, DNA, and proteins from a single sample. Using TRIzol ™ After homogenizing the sample with reagents, chloroform was added to separate the homogenate into a transparent upper water layer (containing RNA), an intermediate phase, and a red lower organic layer (containing DNA and protein). Use isopropanol to precipitate RNA from the aqueous layer. Use ethanol to precipitate DNA from the intermediate phase/organic layer. Proteins were precipitated from the ethanol supernatant by isopropanol precipitation. Wash the precipitated RNA, DNA, or protein to remove impurities, and then resuspend for downstream applications.
For Research Use Only. Not for use in diagnostic procedures.
For scientific research purposes only
