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Shanghai Yingdong Biotechnology Co., Ltd
Sigma monoclonal anti FLAG&# 174; M2 mouse anti
NegotiableUpdate on 05/06
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Overview
Sigma monoclonal anti FLAG M2 mouse antibody can bind to affinity purified monoclonal antibodies containing FLAG peptide sequences. This antibody can recognize the N-terminus, Met-N-terminus, C-terminus of the FLAG peptide sequence and internal sites of the fusion protein.
Product Details
Biological source
mouse
Conjugate
unconjugated
Antibody form
affinity isolated antibody
Antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Purification method
affinity chromatography (affinity tag purification)
Species reactivity
all
concentration
1 mg/mL
Isotope/Subtype
IgG1
Immunogen sequence
Dykddddk
transportation
wet ice
Storage temperature
−20°C
General description
Mouse anti-FLAG M2 antibody can bind to affinity purified monoclonal antibodies containing the FLAG peptide sequence. This antibody can recognize the N-terminus, Met-N-terminus, C-terminus of the FLAG peptide sequence and internal sites of the fusion protein.
Purification method - affinity tag purification
Purification method - affinity tag purification
specificity
Binding site: N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Immunogen
FLAG； Peptide sequence DYKDDDDK
application
Used for immunoblotting[1]Immunoprecipitation (IP)[2][3][4]Immunohistochemistry, immunofluorescence[5]High sensitivity and specificity detection of FLAG fusion protein using immunohistochemistry. Optimize single band detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Protein immunoblotting and EIA
For more product information, please visit our FLAG®Application page.
Protein immunoblotting and EIA
For more product information, please visit our FLAG®Application page.
appearance
Contains 50% glycerol in phosphate buffer solution.
Preparation instructions
Dilute 3% skim milk in Tris buffered saline solution (TBS) at pH 8.0 before immediate use
