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Sigma Anti-FLAG&# 174; M2 affinity gel

NegotiableUpdate on 05/06
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Overview

Sigma Anti-FLAG #174; M2 affinity gel is a mouse monoclonal antibody that can be covalently linked with agarose. This antibody can bind to FLAG at the N-terminus, Met-N terminus, C-terminus, and internal positions of the fusion protein. Combination is not dependent on calcium.

Product Details

Conjugate

agarose conjugate

Antibody form

purified immunoglobulin

Antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

Chemical category of analyte

proteins

technology

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

(4% agarose bead; 45-165μm bead size)

Isotope/Subtype

IgG1

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

transportation

wet ice

Storage temperature

−20°C

General description

Anti-FLAG M2 affinity gel is a mouse monoclonal antibody that can be covalently linked with agarose. This antibody can bind to FLAG at the N-terminus, Met-N terminus, C-terminus, and internal positions of the fusion protein. Combination is not dependent on calcium.

Elution product - FLAG®肽, pH3.5,3x FLAG®peptide
FLAG®peptide, Glycine, pH3.5, 3x FLAG®peptide

Immunogen

Dykddddk

application

Anti FLAG®M2 affinity gel has been used for western blotting, immunoprecipitation and purification of FLAG fusion protein.[1][2][3]

Please visit our FLAG®Apply the portal website to learn more about product details.

appearance

Suspended in a buffer salt solution containing azide as a preservative and 50% glycerol